Maize Genotypic Variation

Maize genotypic variation is the heritable genetic diversity among maize lines, varieties, and populations, and it helps explain why plants differ in growth, development, and productivity. Differences in alleles and gene regulation influence traits such as flowering time, plant architecture, root development, and stress responses, while environmental conditions determine how those traits are expressed. Researchers compare genotypes across locations or controlled treatments to measure genotype-by-environment interactions, distinguishing broadly adapted materials from those suited to particular climates, soils, or management systems. This knowledge supports breeding for yield stability, drought and heat tolerance, resource-use efficiency, and resilience as growing conditions change.

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Education

JoVE Science Education - Advanced Biology
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SNP Genotyping

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2023

Single nucleotide polymorphisms, or SNPs, are the most common form of genetic variation in humans. These differences at individual bases in the DNA often do not directly affect gene expression, but in many cases can still be useful for locating disease-associated genes or for diagnosing patients. Numerous methodologies have been established to identify, or “genotype”, SNPs.JoVE’s introduction to SNP Genotyping begins by discussing what SNPs are and how they can be used to identify...

Research

JoVE Journal - Biology

Lateral Root Inducible System in Arabidopsis and Maize

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Cited by 8 •

2016

The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.

Mouse Genotyping

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2023

Even though the human genome was mapped over 10 years ago, scientists are still far from understanding the function of every human gene! One way to evaluate how a gene functions is to disrupt the sequence encoding it and then evaluate the impact of this change (the phenotype) on the animal’s biology. This approach is commonly used in the mouse (Mus musculus), since it shares a high degree of genetic similarity with humans. To track the animals bearing genetic changes over several generations,...

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JoVE Journal - Biology
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Scalable Transfection of Maize Mesophyll Protoplasts

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Cited by 10 •

2023

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

Research

JoVE Journal - Biology
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Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

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Cited by 1 •

2023

Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal imaging.

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