Biological Tissue Imaging
Biological Tissue Imaging - Related Videos
06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
0 Views •
Cited by 21 •
2013
Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue sections.
09:02
SEM Imaging of Biological Samples
0 Views •
2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut A scanning electron microscope (SEM) is an instrument that uses an electron beam to nondestructively image and characterize conductive materials in a vacuum. As an analogy, an electron beam is to the SEM as light is to the optical microscope. The difference is that the electron microscope yields images of much higher resolution and magnification. The...
09:06
In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
0 Views •
Cited by 5 •
2021
Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.
09:22
Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
0 Views •
Cited by 29 •
2010
Laser ablation electrospray ionization (LAESI) is an atmospheric-pressure ion source for mass spectrometry. In the imaging mode, a mid-infrared laser probes the distributions of molecules across a tissue section or a biofilm. This technique presents a new approach for diverse bioanalytical studies carried out under native experimental conditions.
07:46
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
0 Views •
Cited by 7 •
2016
ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.
FAQs
Contrast depends on how tissue absorbs, scatters, emits, or alters an applied signal. Optical, acoustic, magnetic, and X-ray-based approaches therefore reveal different aspects of tissue structure, composition, or function. Selecting a signal interaction that responds to the feature of interest helps distinguish cellular organization, material distribution, or organ-scale architecture within the resulting image.
Biological tissue imaging can resolve features from individual cells to whole organs, so the useful method depends on the scale of the biological question. Cellular organization may require fine structural or molecular contrast, whereas organ-level assessment emphasizes broader architecture and function. Matching scale and contrast to the research objective improves interpretation across engineered tissues and disease models.
Structural contrast shows how tissue is organized, while molecular contrast can reveal differences in composition or biological activity. Combining these perspectives allows investigators to relate visible architecture to tissue state rather than relying on appearance alone. This distinction is important when evaluating whether engineered tissues develop appropriate organization, composition, and functional characteristics.
A study typically begins by identifying the tissue feature or function to assess, then selecting an optical, acoustic, magnetic, or X-ray-based signal suited to that goal. The signal is applied and the tissue response is detected to generate images. Researchers can then measure structural, compositional, or functional features and relate them to biological or mechanical performance.
In bioengineering, imaging supports characterization of engineered tissues and monitoring of scaffold integration. It can show whether cells are organized within a construct, whether vascularization develops, and whether cells remain viable. These observations help researchers compare biomaterials and tissue models, identify changes during development, and assess whether a construct is progressing toward its intended biological performance.
Quantitative imaging converts tissue features into measurements that can connect architecture with mechanical or biological performance. Such measurements help validate tissue models, refine biomaterials, and evaluate regenerative strategies. The same approach also supports disease studies by providing information about changes in tissue structure, composition, or function across relevant scales rather than relying only on qualitative visual inspection.