Desolvation System
Desolvation System - Related Videos
12:10
Microinjection of Xenopus Laevis Oocytes
0 Views •
Cited by 14 •
2009
Here we demonstrate cytoplasmic microinjection of Xenopus laevis oocytes with a nuclear import substrate, as well as preparation of the injected oocytes for visualization by thin-sectioning electron microscopy.
09:22
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
0 Views •
Cited by 12 •
2015
We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.
09:20
Fertilization of Xenopus oocytes using the Host Transfer Method
0 Views •
Cited by 13 •
2010
Procedure for fertilizing Xenopus oocytes by the host transfer method.
07:06
Visualizing RNA Localization in Xenopus Oocytes
0 Views •
Cited by 5 •
2010
Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.
10:24
Xenopus laevis as a Model to Identify Translation Impairment
0 Views •
Cited by 2 •
2015
Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.
FAQs
Controlled heating promotes evaporation of the volatile solvent, while gas flow helps carry that vapor away from the remaining sample material. Condensation provides a corresponding route for separating the removed solvent. Together, these functions reduce the liquid burden delivered downstream while preserving less volatile dissolved analytes for subsequent measurement or processing.
The separation depends on a difference in volatility between the solvent and the dissolved constituents. A volatile solvent can be removed through evaporation, whereas less volatile biological or chemical analytes remain available for downstream transfer. This distinction allows the system to reduce liquid volume without intentionally removing the proteins, metabolites, nanoparticles, or other materials being analyzed.
Reducing solvent load can make the downstream process more stable by limiting the amount of liquid entering the analytical system. In the workflows described, this reduction can also decrease interference and support more sensitive measurements. The practical value is improved handling of concentrated sample material before aerosol-based spectroscopy, mass spectrometry, or biomolecule characterization.
A liquid sample or aerosol droplets first encounter controlled conditions that encourage solvent evaporation. Gas flow assists removal of the resulting vapor, while condensation supports separation of the solvent from the retained sample constituents. The partially or substantially desolvated material then proceeds into a downstream analytical or processing workflow with less solvent accompanying it.
The key conditions identified for this process are controlled heating and gas flow, because both influence how efficiently solvent is removed from liquid samples or aerosol droplets. Their coordinated use determines how much solvent reaches the next stage while supporting retention of less volatile constituents. These conditions are therefore important when preparing samples for sensitive analytical measurements.
Bioengineering researchers may use this component when liquid samples or aerosolized materials must enter an analytical workflow with reduced solvent burden. Relevant applications include aerosol-based spectroscopy, mass spectrometry, and biomolecule characterization. The approach is especially useful for workflows involving proteins, metabolites, nanoparticles, and other biological materials whose measurement can benefit from improved sample transfer and concentration.
Researchers can assess whether the reduced solvent load improves signal stability, limits interference, and supports more sensitive measurements. These outcomes matter when characterizing biological or chemical constituents after solvent removal. In bioengineering workflows, the resulting sample condition can affect the quality of information obtained about proteins, metabolites, nanoparticles, and other biomolecules.