Polypeptide Coating
Polypeptide Coating - Related Videos
07:35
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
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Cited by 47 •
2014
Elastin-like polypeptides are stimulus-responsive biopolymers with applications ranging from recombinant protein purification to drug delivery. This protocol describes the purification and characterization of elastin-like polypeptides and their peptide or protein fusions from Escherichia coli using their lower critical solution temperature phase transition behavior as a simple alternative to chromatography.
09:42
Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
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Cited by 6 •
2012
We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.
10:15
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Cited by 2 •
2012
A technique to identify translational pause sites on mRNA is described. This procedure is based on isolation of nascent polypeptides accumulating on ribosomes during in vitro translation of a target mRNA, followed by the size analysis of the nascent chains using a denaturing gel electrophoresis.
13:21
Graphene Coatings for Biomedical Implants
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Cited by 37 •
2013
Graphene offers potential as a coating material for biomedical implants. In this study we demonstrate a method for coating nitinol alloys with nanometer thick layers of graphene and determine how graphene may influence implant response.
11:34
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
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Cited by 2 •
2022
We present a protocol to screen anti-hepatitis B virus (HBV) compounds targeting pre- and post-viral entry lifecycle stages, using isothermal titration calorimetry to measure binding affinity (KD) with host sodium taurocholate cotransporting polypeptide. Antiviral efficacy was determined through the suppression of viral lifecycle markers (cccDNA formation, transcription, and viral assembly).
FAQs
These properties determine which chemical features remain exposed at the material interface and how the layer presents them to the surrounding biological environment. Sequence can provide different functional groups, charge affects electrostatic interactions, and conformation controls their accessibility. Together, they influence surface hydrophilicity, molecular binding, and the way cells or biomolecules interact with the coated material.
Exposed functional groups create interaction sites at the surface rather than leaving the underlying material as the only biological contact point. Their presence can change how water, biomolecules, and cells associate with the interface. In practice, this allows researchers to adjust molecular binding and cellular responses by controlling which chemical features the coating presents.
The attachment method helps determine how the polypeptide layer is positioned and maintained at the material surface. Because orientation and accessibility influence which sequence features remain available, attachment can affect biochemical cue presentation and interactions with cells or biomolecules. Selecting this aspect alongside sequence and charge is therefore important when designing a specific interface.
Design begins with the intended biological interaction, then considers the polypeptide sequence, charge, conformation, and attachment method. These variables should be matched to whether the surface must alter hydrophilicity, support molecular binding, or present biochemical cues in a controlled way. The same design logic can be adapted for culture substrates, biosensors, or device surfaces.
On culture substrates, the coating can modify the interface encountered by neural cells and provide a surface suited to studying cell-material interactions. Its composition and presentation can be selected to support neuronal adhesion, neurite growth, or controlled biochemical cue display. This makes the approach useful when the substrate itself needs to contribute to the experimental environment.
Neural devices and implantable research tools contact biological tissues and molecules through their surfaces, so interface properties can influence compatibility. A polypeptide layer offers a flexible way to modify that boundary and present selected biochemical features. In neuroscience research, this supports efforts to improve neural interfaces, biosensors, and other tools used around neural systems.
These coatings allow researchers to examine how changes in a material surface affect neuronal adhesion, neurite growth, molecular binding, and biochemical cue presentation. Comparing different sequences, charges, conformations, or attachment methods can reveal which interface features produce particular responses. The resulting observations help connect surface design with cell-material interactions and neural device performance.