Nonlinear Optical Imaging
Nonlinear Optical Imaging - Related Videos
07:13
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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Cited by 1 •
2022
This article presents the integration of a spectral-focusing module and a dual-output pulse laser, enabling rapid hyperspectral imaging of gold nanoparticles and cancer cells. This work aims to demonstrate the details of multimodal nonlinear optical techniques on a standard laser scanning microscope.
15:06
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
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Cited by 2 •
2016
Saturable and reverse saturable scattering were discovered in isolated plasmonic particles and adopted as a novel non-bleaching contrast method in super-resolution microscopy. Here the experimental procedures of detecting and extracting nonlinear scattering are explained in detail, as well as how to enhance resolution with the aid of saturated excitation microscopy.
01:22
Nonlinear Pharmacokinetics: Causes of Nonlinearity
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2025
Nonlinearity in drug pharmacokinetics is caused by various factors influencing how a drug is absorbed, distributed, metabolized, and excreted. Understanding these nonlinear processes is crucial for predicting drug behavior in the body and optimizing drug dosing regimens. Nonlinear drug absorption can occur when the process is rate-limited by solubility, carrier-mediated transport systems, or saturation of the presystemic gut wall or hepatic metabolism. For instance, high doses of riboflavin...
08:53
Mesoscopic Optical Imaging of Whole Mouse Heart
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Cited by 6 •
2021
We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
FAQs
Two-photon excitation uses a tightly focused pulsed laser to drive simultaneous photon absorption. Because the response depends nonlinearly on illumination intensity, excitation is concentrated near the beam focus. This creates a localized signal and supports three-dimensional imaging with less out-of-focus excitation, which is useful for examining living cells and engineered tissues in place.
Second- and third-harmonic generation produce contrast by converting tissue properties into signals at new wavelengths, whereas two-photon excitation relies on simultaneous photon absorption. These harmonic-generation responses can provide information about tissue organization without relying exclusively on added labels. That distinction is valuable for studying engineered tissues and extracellular matrix structure with limited sample preparation.
These methods can generate image contrast from optical responses of the sample itself, so imaging may be label-free or minimally labeled. That characteristic reduces dependence on extensive sample preparation and supports observation of living cells, engineered tissues, and extracellular matrix organization. It also helps bioengineers examine native structure alongside changes associated with biological or material behavior.
Two features are central: tightly focused illumination and pulsed laser delivery. Focusing confines the interaction to a defined region, while pulsed illumination provides conditions needed for nonlinear responses such as two-photon excitation. Together, these conditions support optical sectioning and three-dimensional imaging rather than a signal dominated by the entire illuminated volume.
Within bioengineering, the approach supports analysis of living cells, engineered tissues, extracellular matrix organization, and dynamic biological processes. Its combination of three-dimensional imaging and reduced out-of-focus excitation allows researchers to study biological structures and material behavior in relevant sample settings. This makes it useful when extensive preparation could interfere with the system being examined.
The resulting images can help researchers evaluate structure, function, and material behavior. Structural information may concern cells, engineered tissues, or extracellular matrix organization, while time-dependent imaging can support investigation of dynamic biological processes. Because the methods can be label-free or minimally labeled, these observations can be made without extensive sample preparation.