Organoid Engineering
Organoid Engineering - Related Videos
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 38 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
10:49
Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening
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Cited by 1 •
2025
Here, we outline a comprehensive protocol for generating and utilizing laboratory-engineered glioblastoma organoids (LEGO) to investigate genotype-phenotype dependencies and screen potential drugs for glioblastoma treatment.
08:45
Tissue Engineering of the Intestine in a Murine Model
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Cited by 29 •
2012
This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.
07:19
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
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Cited by 18 •
2020
This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.
07:42
A High-Throughput Platform for Culture and 3D Imaging of Organoids
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Cited by 4 •
2022
This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.
FAQs
Extracellular matrix materials provide a three-dimensional environment that supports cell organization and tissue architecture. In combination with controlled culture conditions, they help stem or progenitor cells respond to growth factors and other cues that guide differentiation and self-organization. Their use is important because the surrounding material can help engineered structures develop tissue-like features rather than remain as simple cell layers.
Defined growth factors and physical or biochemical cues direct how cells differentiate, organize, and develop tissue architecture. These inputs help researchers control aspects of organ development and function within the engineered system. Adjusting such signals can therefore influence the resulting organoid’s biological characteristics, making them central variables when modeling development, disease mechanisms, or responses to treatments.
Organoids reproduce three-dimensional tissue-like organization, whereas many two-dimensional cultures provide a flatter cellular environment. This spatial organization can represent aspects of human organ development and function more closely, giving researchers another model for studying biological processes. The distinction is especially relevant when investigating disease mechanisms, host-pathogen interactions, or drug responses that may depend on tissue architecture.
A typical workflow begins with stem cells or progenitor cells maintained in controlled culture conditions. Researchers then combine extracellular matrix materials with defined growth factors and selected physical or biochemical cues to guide differentiation and self-organization. Subsequent engineering may incorporate biomaterials, microfluidics, or bioreactor design to improve reproducibility, maturation, vascularization, or scalability for a specific research goal.
Engineered organoids support studies of organ development, disease mechanisms, host-pathogen interactions, and drug responses. Their tissue-like organization allows researchers to examine these questions in systems designed to represent aspects of human biology. Because the models can be shaped by defined culture and engineering conditions, they also provide a platform for comparing biological outcomes under controlled experimental settings.
These engineering approaches address practical limitations in organoid research. Biomaterials help control the three-dimensional environment, while microfluidics and bioreactor design contribute to improved culture control and scalability. Together, such advances are aimed at increasing reproducibility, maturation, and vascularization, supporting broader use in personalized research and regenerative medicine as organoid systems become more consistent and experimentally manageable.