Static Bioreactor
Static Bioreactor - Related Videos
11:11
Construction and Characterization of a Novel Vocal Fold Bioreactor
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Cited by 8 •
2014
A novel vocal fold bioreactor capable of delivering physiologically relevant, vibratory stimulation to cultured cells is constructed and characterized. This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic environment that modulates the behaviors of mesenchymal stem cells.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
11:01
Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue
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Cited by 2 •
2022
The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.
09:46
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
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Cited by 12 •
2017
Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix production.
FAQs
Temperature, pH, oxygen availability, nutrient composition, and incubation time can each affect how cells, tissues, or microorganisms perform in culture. Adjusting these factors gives researchers a defined way to examine changes in viability, growth, differentiation, or product formation. Controlling the variables also helps separate the effects of culture conditions from the biological behavior being studied.
The comparison reveals how a stationary culture environment differs from one shaped by fluid movement or perfusion. Researchers can assess whether operating conditions alter cell viability, tissue development, biomaterial behavior, or product formation. This side-by-side approach is particularly useful when determining which environmental features support a desired biological outcome in bioengineering.
A stationary environment provides a straightforward reference condition for studying biological materials under defined settings. Because researchers regulate factors such as pH, temperature, nutrients, oxygen availability, and incubation time, they can connect observed changes to specific culture conditions. This makes the system useful for evaluating growth, differentiation, tissue development, and other biological responses.
Researchers should define the culture conditions relevant to their biological question, including temperature, pH, oxygen availability, nutrient composition, and incubation time. Maintaining these factors as intended supports consistent observation of cell, tissue, or microorganism behavior. The selected conditions should match the outcome being evaluated, such as viability, expansion, differentiation, tissue development, or product formation.
This platform is useful when researchers need to examine cell expansion, tissue development, or the performance of a biomaterial under controlled culture conditions. It can provide a practical setting for evaluating how cells or tissues respond over an incubation period. Results may help identify conditions associated with improved viability, growth, differentiation, or biological material formation.
Experiments can show how defined culture conditions influence viability, growth, differentiation, and product formation. In bioengineering, these measurements support assessment of cell expansion, tissue development, and biomaterial performance. When results are compared with those from dynamically operated systems, they can also clarify which aspects of the culture environment are associated with particular biological outcomes.