The eye is a complex organ with varied tissue composition. A variety of disorders resulting in vision impairment and reduced quality of life arise from the involvement of different ocular tissues. Among them, dry eye disease, corneal blindness, glaucoma, cataract, and retinal blindness can be accounted for and effected by the lacrimal gland, corneal damage, trabecular meshwork clogging, lens cloudiness, and retinal cell impairments, respectively. The available treatment strategies involving therapeutics and surgery may have limited outcomes, especially in reversing vision deterioration. When the current treatment approaches fall behind, tissue regeneration approaches come into play to improve and help restore vision. Regenerative medicine approaches are versatile and difficult to design and implement and require further ingenuity when the specific organ is a very complex one such as the eye.

This methods collection highlights the necessity and applicability of tissue regeneration strategies for ocular tissues. Invited methods in tissue engineering include the selection of scaffold material, scaffold design, 3D printing options, and cell-sheet engineering approaches. Additionally, organoid and lab-on-a-chip development methods for the investigation of new treatment strategies as well as tissue replacement therapies are invited. As key milestones of regenerative medicine, protocols describing the isolation and in vitro culture methods for ocular primary cells and stem cells are welcome.