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TOPICAL COLLECTIONS

Methods for the Detection and Quantification of Autophagy

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Claire Gordy

Claire Gordy

North Carolina State University

Claire Gordy is an Assistant Teaching Professor in the Department of Biological Sciences at North Carolina State University. Her research focuses on cultivating inclusive learning environments in the college biology classroom through the incorporation of tactile teaching tools and the development of authentic course-based undergraduate research experiences (CUREs). Through an Elementary Genetics CURE at NCSU, she co-mentors 240 undergraduate researchers working to characterize stress tolerance phenotypes in novel wild yeast strains each academic year. This work has involved the development of straightforward techniques for quantitative mid-throughput screening of genetic fitness that can be consistently performed by beginning undergraduate researchers. Outside of the classroom, her research focuses on understanding connections between autophagy and other cellular responses. Prior to her current position, she studied connections between the G protein-coupled receptor-mediated pheromone pathway and autophagy in S. cerevisiae as a post-doctoral researcher in the Department of Biochemistry at the University of North Carolina - Chapel Hill, and the roles of autophagy and apoptosis in the development and homeostasis of the immune system as a PhD student in the Department of Immunology at Duke University.

Collection Overview

Autophagy is a cellular self-eating process conserved from yeast to humans. Once considered an oddity of yeast cells, autophagy is now known to promote cell survival and regulate processes ranging from immunity and cancer in higher eukaryotes, to development and senescence in both higher and lower eukaryotes. The phenomenon broadly referred to as “autophagy” is composed of two separate processes: macroautophagy and microautophagy. Moreover, autophagy can be used to recycle bulk cytoplasmic contents; to specifically degrade aged, damaged, or excess organelles; or to clear intracellular pathogens. Thus, unraveling the function of autophagy in organismal homeostasis, development, and disease requires a full complement of assays to detect and quantify autophagic initiation and flux. This collection will not only include traditional methods; such as electron microscopy-based detection of autophagosomes, Western blot-based detection of LC3/Atg8 processing and degradation of organelle-specific markers, and fluorescence microscopy-based quantification of LC3/Atg8 puncta, but will also focus on novel strategies that allow detection of autophagy in live cells, quantitative measures of autophagy, and high throughput screening of autophagy-related phenotypes.

 

 

Articles

Western Blot Analysis of Microtubule-Associated Protein Light Chain 3 to Monitor Autophagosomal Dynamics in Mouse-Derived Pancreatic Cancer Cells

Western Blot Analysis of Microtubule-Associated Protein Light Chain 3 to Monitor Autophagosomal Dynamics in Mouse-Derived Pancreatic Cancer Cells

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2026

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Abstracts

Detection of mitochondrial autophagy in adipocyte differentiation

Zhiyong Cheng*1

1University of Florida

WIPI β-propeller proteins as autophagy biomarkers

Tanya Pareek 1,

Tassula Proikas-Cezanne*1

1Institute for Cell Biology, Department of Biology, Faculty of Science, Eberhard Karls Unoiversity Tuebingen, Tuebingen, Germany.