The lymphatic vascular system is part of the circulatory system made of a network of vessels similar to the blood vascular system. It acts as the main transport pathway of fluids and elements that contain lymph from the parenchymal tissues to the nodes via the afferent lymphatic and from the nodes back to the venous blood via the efferent lymphatics. Lymphatic dysfunction can lead to conditions such as lymphedema, where the lymphatics are damaged or blocked, and are also related with immune conditions, such as inflammatory bowel disease, where the immune system influences the lymphatics to affect their function. Basic science research has increased our overall knowledge of the lymphatic system, though there remains to be a paucity of data of the lymphatic systems role in physiological and pathophysiological conditions. Various basic science experimental models and techniques provide us with a platform to increase our knowledge of the lymphatics. This collection aims to exhibit modern techniques that benefit lymphatic researchers to study the physiological and pathophysiological changes with the lymphatic system. It is critical from an experimental approach to determine what is altered in the living animal, and there have been various approaches to measure lymphatic adaptations from living samples. These include ex vivo functional tests of lymphatic vessels by pressure myography, injectable tracer systems selectively up-taken by lymphatics vessels and non-invasively imaged, and live-cell imaging of lymphatic vessels for markers such as calcium. There also exist various techniques to study lymphatic structural and biochemical adaptations (e.g., immunofluorescence).