The development of various diseases involves a dynamic interplay among not just resident cells, but also cells recruited from the circulation. Myeloid cells are derived from hematopoietic precursors and migrate to sites of interest where they play a role in modulating all stages of disease formation. However, while there is mounting evidence about the importance of myeloid cells, much of myeloid cell biology and cell interactions remains incompletely misunderstood.This collection aims to provide new methods for the isolation, differentiation, and utilization of myeloid cells for therapy and regeneration. The ability to collect and isolate myeloid cells from either human or animal tissue and encourage differentiation into different myeloid subtypes is critical to understand the function of various myeloid subpopulations. Furthermore, the ability to obtain these cells and then further use them for therapeutic purposes will also serve as important methods in this collection. These peer-reviewed methods will improve the understanding of myeloid cell biology, which could have important implications for healing after injury, immunotherapies for cancer, or other emerging cell-based therapies.