Salivary gland damage as a result of radiotherapy for the treatment of head and neck cancers or due to autoimmune Sjögren’s syndrome remains a significant problem that causes xerostomia (sensation of dry mouth) and hyposalivation that can result in several oral complications (e.g., increased incidence of oral infections and dental caries, changes in saliva quality, and difficulties with swallowing and speech). A variety of approaches have been taken to regenerate salivary glands, including several biomaterials and tissue engineering approaches that are currently being investigated and optimized in pre-clinical animal models. Single- and double-layer cell sheets, hydrogels containing a variety of factors, and polymer scaffolds are a few of the many biomaterials approaches. This methods collection aims to highlight the biomaterials approaches for salivary gland regeneration and the models and techniques used for these studies.