Despite well-developed pharmacological and interventional treatments, cardiovascular diseases remain the leading cause of mortality in the industrialized world. Vascular or endothelial injury is a crucial mediator in almost all cardiovascular diseases. The two most common causes of vascular injury are hypertension and oxidative stress. One of the gravest consequences of vascular injury is myocardial infarction (which can lead to acute or chronic heart failure), this, in turn, results in an increased disability and mortality burden to society. Reasons for such high mortality rates may lay in an incomplete understanding of the biology of vascular injury, which can hinder both primary and secondary prevention efforts. This collection will aim to present methods that are employed in studying possible pharmacological or nutritional treatments as well as the biology of hypertension, vascular injury, and myocardial infarction. These methods will include novel, less invasive surgical techniques used for myocardial infarct induction in rodent models. Such models are quite suitable for studying myocardial infarction related inflammation and healing, in addition to myocardium remodeling and the development of acute and/or chronic heart failure. Furthermore, this collection will include gold standard methods of the ex vivo Langendorff isolated heart perfusion technique through which the contractile properties of the heart can be studied. Following that, an ex vivo assay of aortic rings will be presented; in this assay, vasomotor effects of many substances can be easily screened. For studying the biology of vascular injury in human subjects arterial stiffness measurement, as one of the noninvasive means of studying endothelial dysfunction, along with flow cytometric analysis of endothelial progenitor cells and plasma microparticles will be presented.