Innovation in minimally invasive surgery (MIS) has dramatically improved the treatment strategies of gastroenterology over the last several decades, allowing doctors to perform many types of complex procedures. Robotic surgery is usually associated with MIS. Robotic surgery supports various types of complex procedures with more precision, flexibility, and control. As a field and platform, gastrointestinal endoscopy has also been established as a therapeutic and other advanced interventional procedure and technique. They represent a dynamic area of ongoing research and have become a fundamental part of the multidisciplinary clinical management of benign and malignant diseases in gastroenterology. Recently, regenerative medicine and surgery have become an expanding branch of translational research in tissue engineering. The growing armamentarium of MIS, endoscopic treatment, and regenerative medicine for various clinical disorders holds exciting potential and opportunities for further innovation and collaboration. This Methods Collection is intended to address the evolving arena of gastrointestinal surgery, endoscopy, and regenerative medicine to showcase and promote the dissemination of recent advances. Prospective authors are interested in submitting manuscripts examining the current state of the art in gastrointestinal treatment, especially those related to the above-mentioned topics.