Arachnids are an incredibly diverse class of terrestrial invertebrates that includes spiders, ticks, mites, harvestmen, scorpions, pseudoscorpions, and whip scorpions, among others. Members of class Arachnida are predators with four pairs of legs and two body segments. They have evolved remarkable abilities of interest to biological research, including animal architecture and a variety of prey capture strategies. In addition, investigating certain arachnid traits could have direct applications for humans, such as venom-derived pharmaceuticals and biomaterials inspired by spider silk. Furthermore, some arachnids like ticks are vectors of human diseases such as Lyme disease and tick-borne encephalitis, and the incidence of these diseases is on the rise. Despite the wealth of biological and biomedical research opportunities arachnids present, few readily available, easily accessible, and standardized methods exist for their rearing and study in a laboratory setting. The goal of this JoVE Methods Collection is to present an array of current molecular, genetic, electrophysiological, and other techniques being applied to arachnids in order to further research into their unique biology.