This Methods Collection aims to gather research dealing with novelties in the field of ophthalmology, both from a diagnostic and therapeutic point of view. Original research is welcome in all subspecialties of ophthalmology, namely strabismus/pediatric ophthalmology, glaucoma, neuro-ophthalmology, retina/uveitis, anterior segment/cornea, oculoplastics/orbit, and ocular oncology. Innovative diagnostics can include imaging, laboratory, and clinical techniques. Therapy-related articles cover advancements in the entire field of ophthalmology as well as outstanding clinical outcomes. All JoVE articles require a step-by-step technique that can be demonstrated in a video. As a result, we welcome any method articles (including clinical and surgical procedures) or original research.