At the crossroads of scientific inquiry and public health protection, toxicology is vital for comprehending and mitigating the diverse chemical hazards that pervade our environment. As society grapples with evolving challenges, including emerging pollutants, climate-related impacts, and rapidly evolving technologies, advancing toxicological research methodologies becomes increasingly paramount.  

In recent years, diverse model organisms have made substantial contributions to this field, particularly in toxin identification and unraveling toxicological mechanisms. Classical mammalian models, such as rodents, and non-mammalian counterparts like zebrafish and nematodes, have joined emerging three-dimensional (3D) model systems like organoids, all of which have become invaluable resources in toxicological research. Moreover, the progression of cutting-edge technologies, including single-cell RNA sequencing and the CRISPR-Cas9 system, opens up additional avenues for elucidating toxicological mechanisms.  

This Methods Collection aspires to provide state-of-the-art methodologies for investigating toxicology in vitro and in vivo, equipping researchers with the means to effectively confront emerging threats.