We think about signaling and the first thing that comes to our mind is kinases and phosphatases. In nature all organisms from prokaryotes to eukaryotes respond to signaling through some type of kinase. Kinases are like the first responders to detect any change in the environment and phosphatases act as the balance by keeping the kinase signaling in check. Kinases are broadly classified into three types based on their substrate specificity- Serine/threonine kinases, Tyrosine kinases and Histidine kinases. Phosphatases are also categorized based on their substrate affinity as Serine/threonine phosphatases and tyrosine-phosphatases. But there also exist a subtype of tyrosine phosphatases called dual-specificity phosphatases.

This method collection page aims to compile the strategies and method that are being used and developed to study phosphorylation and dephosphorylation in any model either in vitro or in vivo. Detection of phosphorylation has always been tricky but it is one of the most crucial post translational modifications we study in signaling.

We encourage submission of methods used to study phosphorylation and dephosphorylation both in prokaryotes and eukaryotes.