The therapeutic landscape of hematological disorders is undergoing a revolutionary shift with the advent of chimeric antigen receptor (CAR) technology, particularly in the context of differentiating T cells, NK cells, and dendritic cells (DCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs).  

This JoVE Methods Collection aims to encapsulate the state-of-the-art methodologies that enable the precise differentiation and genetic manipulation of these cells, with a focus on the integration of CAR technology to advance personalized immunotherapy. We call upon experts in stem cell research, genetic engineering, and immunotherapy to contribute to this vital compendium. The collection is set to feature detailed visual documentation of protocols that chart the course from pluripotent stem cells to fully functional immune effector cells equipped with CAR constructs.  

Contributions should push the envelope of existing differentiation techniques, with an emphasis on innovative applications of CAR technology in hESC- and iPSC-derived hematopoietic cells. We are particularly interested in novel approaches to optimize CAR-T cell production, advancements in the differentiation and expansion of CAR-NK cells, and strategies for generating DCs with enhanced antigen-presenting capabilities using iPSCs.