Current research has focused on various strategies and technologies to evaluate endogenous regenerative mechanisms and their limits in the onset of irreversible degenerative conditions. Unfortunately, several organs in our body cannot counteract their progressive degeneration, which leads to major neurological, cardiac, bone, and articular diseases.

Researchers from several scientific disciplines have investigated possible methods to unveil the driving forces behind molecular, cellular, and tissue alterations in the affected regions. Indeed, from established principles in developmental biology to advances in molecular biology and tissue engineering, worldwide research is seeking insights into the initiation and progression of tissue degeneration as well as suitable strategies to reverse the pathological condition of interest. 

Therefore, this Methods Collection is aimed to gather multiple contributions which could focus on:  

• Organoid culturing to reproduce molecular pathways and cellular alterations 
• Stem cell-based techniques to obtain tissue regeneration
• Advanced omics and spatial analyses to detect signs of localized degeneration
• Bioprinting and biomaterial scaffolds to study microenvironmental cues 
• Gene editing to dissect specific patterns in defects
• Application of protocols to test the systemic effect of therapeutics 
• Investigation of alterations in the extracellular matrix  
• Mathematical models to stratify pathological conditions and predict their possible reversal