The present collection aims to highlight innovative methodologies that bridge fundamental research and clinical application in the study of osteoarthritis—a highly prevalent, multifactorial joint disease with significant public health implications. As the demand for disease-modifying therapies increases, developing and refining biomedical tools for osteoarthritis research has become more urgent than ever. This collection welcomes contributions that span the translational continuum, including molecular and cellular studies, animal models, imaging techniques, biomarker discovery, and therapeutic development. Emphasis is placed on methodological rigor, reproducibility, and approaches that facilitate the translation of scientific findings into clinical practice. By gathering diverse perspectives and approaches, this collection seeks to promote interdisciplinary collaboration and accelerate progress toward more effective, personalized interventions for osteoarthritis. Suitable topics include, but are not limited to, preclinical models, omics-based approaches, drug screening platforms, and clinical methodologies relevant to osteoarthritis and related musculoskeletal disorders.