Tissue repair remains a key challenge in regenerative medicine. While biochemical signals have been extensively studied, emerging evidence highlights the crucial role of biophysical cues (mechanical forces, electrical signals, matrix stiffness) in regulating cellular behavior and tissue regeneration. These physical cues orchestrate tissue repair by modulating cell migration, proliferation, differentiation, and extracellular matrix remodeling, thereby establishing innovative therapeutic strategies widely adopted in clinical practice.

This Methods Collection aims to explore the molecular mechanisms and clinical applications of biophysical signals in tissue repair and regeneration. The scope includes, but is not limited to:

1. Mechanical signaling in fibrosis and regeneration, including pathological scar formation and skin tissue expansion techniques.

2. Regulation of cellular behaviors by bioelectrical signals and their guidance in wound healing and neural repair.

3. Effects of matrix mechanical properties (e.g., stiffness, topography) on stem cell fate determination and their implications for biomimetic material design.

4. Optimization and future direction of clinical biophysical therapeutic technologies.

We aim to bridge fundamental research and clinical applications, fostering interdisciplinary innovation in tissue engineering and regenerative medicine.