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TOPICAL COLLECTIONS

Liquid Biopsy Revolution: Advancing Personalized Tumor Diagnosis and Monitoring
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Chiara Nicolazzo

Chiara Nicolazzo

Department of Life Science, Health and Health Professions, Link Campus University, Rome, Italy Cancer Liquid Biopsy Unit, Department of Experimental Medicine, Sapienza University, Rome, Italy

Chiara Nicolazzo is an Associate Professor at the Department of Life Science, Health and Health Professions at Link Campus University of Rome, Italy. She received her Master’s degree in Pharmaceutical Chemistry and Technologies, a PhD in Molecular Medicine, and a specialization in Clinical Pathology and Clinical Biochemistry from Sapienza University.Since 2008, her research has focused on cancer biology, particularly on the study of tumor-derived biomarkers circulating in the bloodstream and other body fluids. Her scientific activity involves the isolation and molecular characterization of circulating tumor cells (CTC) and the analysis of circulating tumor DNA (ctDNA), with a particular emphasis on colorectal cancer. She is strongly committed to overcoming technical challenges in the field of liquid biopsy and to the development and optimization ofintegrated methods for CTC and ctDNA analysis from a single blood sample. She is actively engaged in promoting translational research in oncology and the development of non-invasive diagnostic strategies.

Paola Gazzaniga

Paola Gazzaniga

Cancer Liquid Biopsy Unit, Department of Experimental Medicine, Sapienza University, Rome, Italy

Paola Gazzaniga is Full Professor of Precision Oncology and Head of the Liquid Biopsy Unit at the Department of Experimental Medicine, Sapienza University of Rome. She began her research career studying HPV and cancer at the Deutsches Krebsforschungszentrum under Prof. Harald zur Hausen. Back in Italy, she focused on cancer biomarkers in blood and was among the first to propose EGFR mutation testing in peripheral blood.A pioneer in liquid biopsy, Prof. Gazzaniga established a dedicated laboratory equipped for the enrichment and molecular characterization of circulating tumor cells (CTCs) and ctDNA. Her group is strongly committed to translating liquid biopsy innovations into clinical tools for patient management.Her research focuses on using liquid biopsies to study clonal evolution in solid tumors, with specific aims including: (1) identifying predictive biomarkers for targeted and immune therapies, (2) early detection of drug resistance, and (3) discovering novel therapeutic targets.

Collection Overview

Liquid biopsy is revolutionizing the field of oncology by offering a minimally invasive window into tumor biology. Through the analysis of circulating biomarkers, such as circulating tumor cells (CTC), circulating tumor DNA (ctDNA), extracellular vesicles (EV), and cell-free RNA, liquid biopsy enables real-time monitoring of tumor evolution, detection of minimal residual disease, and assessment of treatment response. Despite its immense potential, broader clinical implementation still requires robust, standardized methodologies that are both reproducible and clinically meaningful.

This Collection aims to provide a comprehensive, visualized resource for cutting-edge techniques in the liquid biopsy field. It brings together methods for isolating and characterizing a wide range of circulating biomarkers from various body fluids. In addition to molecular profiling approaches, such as targeted sequencing, methylation analysis, and transcriptomics, this Collection emphasizes functional applications, including the development of 3D culture models from CTC and the integration of multi-omic data into personalized medicine frameworks. The Collection will also address pre-analytical considerations, such as sample collection, handling, and storage protocols, which are critical for preserving biomarker integrity. By covering both fundamental and translational aspects, this resource will serve not only as a technical reference but also as a catalyst for harmonizing methodologies and accelerating clinical adoption.

Articles

Robust Isolation and Multiplexed Identification of Circulating Rare Cells <em>via</em> Continuous Centrifugal Microfluidics and Hydrogel Immunocytochemistry

Robust Isolation and Multiplexed Identification of Circulating Rare Cells via Continuous Centrifugal Microfluidics and Hydrogel Immunocytochemistry

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2026

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