Developing embryos are not only used in research in developmental biology and cell biology, but are also utilized as an important tool in biotechnology and cancer research to develop new techniques and to test drugs. Among many model animals, avian embryos, including chick and quail, representatively, provide very easy access to cells, tissues, and organs at various developmental stages because they grow in the egg, out of their mother’s body. Also, there are only minimal requirements for culturing avian embryos, such as appropriate temperature and egg white (in case of early-stage embryos), which increases their availability. This collection will focus on, but is not limited to, various current culture techniques for avian embryos, including in ovo, ex ovo, and other culture methods, as well as associated techniques for analysis with avian embryos. Original research that utilizes these approaches or explores new findings using avian embryo models is also welcome.