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TOPICAL COLLECTIONS

Current Methods in Retrieving Genetic Resources
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Haixing Li

Haixing Li

Sino-German Joint Research Institute, Nanchang University

Dr. Haixing Li joined the Sino-German Joint Research Institute at Nanchang University in China in 2004 and currently serves as a professor there. His research focuses on innovations in PCR and the probiotic production of gamma-aminobutyric acid, the major inhibitory neurotransmitter in mammals.In recent years, Dr. Li's laboratory has developed several PCR-based genome-walking techniques, including wristwatch PCR, bridging PCR, and racket PCR. He received his BSc in Biochemical Engineering from the Dalian College of Light Industry in China in 2001. He earned his master's in Microbiology and his PhD in Food Science and Engineering from Nanchang University in 2004 and 2012, respectively. Following this, he worked as a postdoctoral fellow in Dr. Jed Lampe's laboratory at the University of Kansas Medical Center in the USA from 2015 to 2016.

Collection Overview

Genetic resources are crucial for understanding the diversity of life, including variations between species, biological functions, and disease mechanisms. Heritability and genetic variability play a vital role in maintaining ecological balance. Responsible study and utilization of these resources are essential for advancing science and improving our interactions with the natural world.


Several molecular techniques—such as PCR, genome walking, next-generation sequencing (NGS), and rapid amplification of cDNA ends (RACE)—are widely used to identify and analyze genetic material. These tools are fundamental in many nucleic acid research applications. However, these techniques are often scattered across different laboratories and are not easily accessible in a unified format.


This collection aims to compile and showcase these methods in one centralized location. We welcome both established techniques and innovative new methods. Submissions may include applications such as gene and allele discovery, mutation detection, full-length gene cloning, and transgene site identification.


The collection will use a video-based format to make these methods easier to follow, replicate, and apply. Our goal is to encourage broader use of these techniques and foster innovation through shared expertise.

Articles

Validation of Random Transgene Integration and Expression in <em>Eimeria</em> Parasites

Validation of Random Transgene Integration and Expression in Eimeria Parasites

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2026

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