Bionanomaterials are at the forefront of biomedical innovation, offering opportunities to design systems that integrate multiple functionalities such as structural support, therapeutic action, diagnostic capability, and regenerative potential. With the rapid evolution of nanoscience, it is now possible to create “smart” multifunctional bionanomaterials that actively respond to biological cues, enhance performance, and improve long-term biocompatibility.

This Methods Collection will highlight experimental and analytical approaches that enable the development and application of smart multifunctional bionanomaterials. Specific areas of interest include:

• Synthesis and functionalization of nanostructures (e.g., nanoparticles, nanotubes, nanocomposites)
• Hybrid material design integrating polymeric, inorganic, and biological components
• Characterization methods for degradation, protein–corona interactions, and immune responses
• Advanced imaging and spectroscopy, including real-time in situ electron microscopy and nanoscale spectroscopy
• Biomedical applications, such as drug delivery systems, antimicrobial surfaces, tissue engineering scaffolds, bioelectronic platforms, and responsive implantable devices

By gathering methodologies from interdisciplinary groups, this collection will serve as a robust resource for reproducible, scalable, and innovative techniques. The overall goal is to accelerate the translation of smart multifunctional bionanomaterials from the laboratory to clinical and industrial applications.