Multimodal microfluidic technologies are rapidly transforming biomedical research by enabling integrated, high‑precision manipulation of cells, biomolecules, and therapeutic agents within microscale environments, supporting applications that span diagnostics, drug discovery, disease modeling, and personalized medicine. By combining active methods such as electrical, magnetic, acoustic, optical, and thermal control with passive mechanisms based on channel geometry, hydrodynamics, capillary flow, viscosity, and inertial effects, these systems provide versatile platforms capable of performing complex workflows, including sample preparation, separation, concentration, detection, screening, and monitoring, within compact, scalable devices. Each modality presents unique operational ranges, sensitivities, integration challenges, and application constraints that can make it difficult for researchers outside the microfluidics community to select the most suitable approach for a given diagnostic or therapeutic need.

Therefore, this Methods Collection seeks submissions that detail the development, optimization, benchmarking, and translational application of multimodal microfluidic systems, including hybrid active–passive platforms, organ‑on‑chip models, wearable or implantable diagnostic devices, high‑throughput screening technologies, AI‑enhanced autonomous microsystems, and industrial case studies, with the overarching goal of providing comprehensive methodological guidance that enables researchers, clinicians, and industry partners to adopt, evaluate, and advance multimodal microfluidics toward more precise, efficient, and clinically relevant biomedical innovations.