This collection focuses on cutting-edge clinical techniques and research methods in the field of urology. Urological conditions, such as tumors, stones, and functional disorders, have a high incidence rate, and innovations in diagnosis and treatment directly impact patient outcomes and quality of life. However, the translation of many novel technologies from the laboratory to clinical practice lacks standardized methodological guidance, which limits their dissemination and reproducibility. This collection aims to bring together experts in urology to showcase practical approaches, including minimally invasive surgical techniques, novel diagnostic tools, and clinical study designs, through a combination of video and text. The goal is to help clinicians rapidly master key techniques, enhance research quality, and improve translational efficiency. By leveraging JoVE’s visual format, researchers can intuitively replicate procedural steps, shorten the learning curve, and promote standardization and international collaboration in urological clinical research.