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TOPICAL COLLECTIONS

Animal Bioindicators: From Field Sampling to Laboratory Assays and Modern Statistical Inference
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Guest Editors

Dora Bjedov

Dora Bjedov

Department of Biology, Josip Juraj Strossmayer University of Osijek

Dr. Dora Bjedov is an assistant professor at the Department of Biology, Josip Juraj Strossmayer University of Osijek, Croatia. Her research lies at the intersection of ecotoxicology, environmental biomonitoring, and wildlife ecophysiology, with a particular emphasis on birds as sentinels of environmental pollution. She integrates biochemical and cellular biomarkers, trace element and organic pollutant analyses, and advanced statistical and machine-learning approaches to assess pollutant exposure and effects across aquatic and terrestrial ecosystems. She has published in leading international journals and is actively involved in international research collaborations and editorial work in environmental and ecological sciences.

Hani Amir Aouissi

Hani Amir Aouissi

Environmental Research Center (CRE), Annaba

Dr. Hani Amir Aouissi is a Senior Researcher Rank “A” (HDR) at the Environmental Research Center (CRE), Annaba, Algeria, where he leads the Research Results Valorization Department, serves as an elected member of the Scientific Council, and acts as President of the Visibility Committee. His research lies at the intersection of environmental biology, ecotoxicology, biodiversity, and global change, with a particular emphasis on bioindicators, aquatic ecosystems, and environmental health. He has authored over 70 peer-reviewed publications (+1100 citations) and has extensive editorial experience as an academic editor, associate editor, and reviewer for numerous international journals. His work integrates field ecology, laboratory-based biomarker assessment, and advanced statistical modeling, including machine learning approaches, to strengthen environmental monitoring and risk assessment frameworks. Dr. Aouissi’s interdisciplinary expertise supports robust, reproducible methodologies that bridge field data, laboratory assays, and modern quantitative inference in environmental sciences.

Brankica Kartalović

Brankica Kartalović

BioSense Institute

Brankica Kartalović, PhD, is a senior researcher at the BioSense Institute, Novi Sad, Serbia, with over 25years of experience in accredited analytical laboratories. She holds a PhD in environmental protection and an MSc in pharmaceutical engineering. Her research focuses on pesticide residues, phthalates, persistent organic pollutants, and micro/nanoplastics in water, soil, and food. She specializes in the development, validation, and harmonization of advanced analytical methods based on GC-MS, LC- MS/MS, and HPLC-DAD/FLD for complex environmental and food matrices. Her work integrates ISO/IEC 17025 quality standards, risk assessment principles, and EU regulatory frameworks, contributing to food safety, environmental monitoring, and sustainable agricultural systems.

Collection Overview

Animals have long served as sentinels of environmental change, quietly integrating chemical, physical, and biological stressors into measurable biological responses, yet the methodological bridge between field observation, laboratory analysis, and data interpretation often remains fragmented. This Topical Collection aims to consolidate and modernize methodologies for animal bioindicators by explicitly positioning them at the interface of ecology, toxicology, veterinary science, and data science, and by showcasing protocols that span the full research pipeline, from robust and ethical field sampling, through classical and emerging laboratory biomarkers, to contemporary statistical frameworks capable of handling complex, multilevel biological data. A central emphasis is placed on methodological comparability and scalability, ensuring that approaches can be meaningfully applied across species, ecosystems, and monitoring frameworks, from local research studies to large-scale regulatory programs.


By combining well-established techniques refined with new technologies alongside novel methodological approaches, this collection will provide reproducible, visually guided protocols that improve data quality, comparability, and ecological relevance. It also encourages cross-sector collaboration between academia, industry, governmental agencies, and non-governmental organizations, ultimately strengthening the role of animal bioindicators in environmental monitoring, risk assessment, and regulatory science.

Articles

Tail Artery Blood Sampling in Isoflurane Anesthetized Rats Reduces Stress and Improves Reliability

Tail Artery Blood Sampling in Isoflurane Anesthetized Rats Reduces Stress and Improves Reliability

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2026

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