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TOPICAL COLLECTIONS

Translational Methods for Diagnosis and Intervention in Aging and Aging-Related Diseases
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Guest Editors

Man Zhu

Man Zhu

Department of Laboratory Medicine, Sichuan University

Dr. Man Zhu is an assistant research fellow in the Department of Laboratory Medicine, West China Hospital, Sichuan University. He received his doctoral degree in medicine from the University of Electronic Science and Technology of China. His research focuses on aging intervention mechanisms, healthspan regulation, and aging-related diseases, with particular interest in brown adipose tissue dysfunction, skeletal muscle aging, sarcopenia, senescence-associated secretory phenotype, epigenetic remodeling, and topoisomerase-mediated molecular regulation.Dr. Zhu has served as the principal investigator for two research projects, including one supported by the Department of Science and Technology of Sichuan Province and one institutional project supported by West China Hospital. He has published more than 10 SCI-indexed articles, including representative studies published in PNAS, Aging Cell, and ACS Nano. By integrating clinical observations with animal models, cellular experiments, and molecular approaches, his work aims to elucidate key mechanisms of aging and identify potential strategies for delaying aging-related functional decline.

Xue Wen

Xue Wen

Department of Plastic and Burn Surgery, Sichuan University

Dr. Xue Wen is an attending physician and postdoctoral fellow in the Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, Chengdu, China. She received her medical doctorate through the eight-year Clinical Medicine Program at Wuhan University. Her research focuses on environmental health, endocrine-disrupting chemicals, epigenetic regulation, adipose tissue dysfunction, and metabolic diseases.By integrating epidemiological studies, animal models, molecular biology techniques, and epigenomic analyses, Dr. Wen investigates how environmental exposures disrupt metabolic homeostasis and contribute to obesity and other chronic diseases. She has served as the principal investigator for one project funded by the National Natural Science Foundation of China, one provincial- or ministerial-level project, and one institutional project supported by West China Hospital. She has published in peer-reviewed international journals, including PNAS, Environmental Pollution, Journal of Molecular Endocrinology, and ACS Nano.

Collection Overview

Aging is a central driver of functional decline across tissues and organs and is closely linked to a broad range of chronic conditions, including metabolic disorders, sarcopenia, neurodegenerative diseases, cardiovascular diseases, impaired wound healing, and frailty. As the burden of aging-related diseases continues to increase, there is a growing need for reliable methods that support earlier diagnosis, functional evaluation, and effective intervention. Despite rapid advances in aging research, the translation of laboratory findings into reproducible diagnostic and therapeutic approaches remains challenging because aging-related diseases involve complex changes in cellular senescence, inflammation, metabolic homeostasis, tissue remodeling, and systemic function.

 

This JoVE collection aims to present reproducible, visually demonstrable, and application-oriented protocols for diagnosis, functional evaluation, and intervention in aging and aging-related diseases. Topics of interest include molecular and imaging-based diagnostic approaches, biomarker detection, cellular senescence and senescence-associated secretory phenotype assays, organ and tissue functional assessment, aging-related animal and cellular models, biomaterials- or nanomaterials-based diagnostic and delivery platforms, and therapeutic strategies targeting metabolic, musculoskeletal, regenerative, or functional decline.

 

By providing step-by-step video protocols and detailed methodological guidance, this collection will help researchers and clinicians standardize experimental procedures, reduce technical variability, and improve the reproducibility and translational impact of aging research. It will serve as a practical resource for investigators developing diagnostic tools, disease models, and intervention strategies for aging biology and aging-related diseases.

Articles

Apoptosis Repressor with Caspase Recruitment Domain in Uncommon Epidermal Growth Factor Receptor-Mutant Lung Adenocarcinoma: An Aging Hypothesis

Apoptosis Repressor with Caspase Recruitment Domain in Uncommon Epidermal Growth Factor Receptor-Mutant Lung Adenocarcinoma: An Aging Hypothesis

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2026

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Abstracts

Prolonged Rewarming Was Associated with Reduced Alkaline Phosphatase Bias in an External Quality Assessment Sample: A Laboratory Study

Dayun Xie1,

Man Zhu*2

1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,

2Department of Laboratory Medicine, West China Hospital, Sichuan University

Hypercalcemia and Hypophosphatemia in a Patient with Gastrointestinal Symptoms: A Diagnostic Case of Primary Hyperparathyroidism

Dayun Xie1,

Man Zhu*2,

Jiao Liu*1

1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,

2Department of Laboratory Medicine, West China Hospital, Sichuan University

Persistent Hypokalemia and Hypomagnesemia Leading to the Diagnosis of Gitelman Syndrome: A Laboratory-Guided Case Report

Dayun Xie1,

Xue Wen2,

Man Zhu*3,

Jiao Liu*1

1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,

2Department of Plastic and Burn Surgery, West China Hospital, Sichuan University,

3Department of Laboratory Medicine, West China Hospital, Sichuan University

Multiwavelength Photometric Correction Reduces Lipemia-Associated Bias in Four Clinical Chemistry Assays: An In Vitro Interference Study

Dayun Xie1,

Wenbiao Zhou1,

Baoxiu Chen1,

Yali Li1,

Xue Wen*2,

Man Zhu*3,

Jiao Liu*1

1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,

2Department of Plastic and Burn Surgery, West China Hospital, Sichuan University,

3Department of Laboratory Medicine, West China Hospital, Sichuan University

Early Nutritional Risk Assessment and Structured Nursing Care in Hemorrhagic Stroke: A Propensity-Matched Cohort Study

Haiyan Zhang1,

Yanzhen Shi*2

1Interventional Medical Center, The Second People's Hospital of Hunan Province (Brain Hospital of Hunan Province), Changsha, Hunan 410007, China,

2Department of Cardiovascular Medicine, The Second People's Hospital of Hunan Province (Brain Hospital of Hunan Province), No. 427, Section 3, Furong Middle Road, Changsha, Hunan 410007, China.

A Tandem Mass Tag-Based Quantitative Urinary Proteomic Workflow for Identifying Candidate Protein Markers in Patients with Gastric Cancer

Yadan Wang1

1Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases

Circulating NT-proBNP and soluble ST2 for Early Cardiac Dysfunction in Older Patients With Acute Exacerbation of Chronic Obstructive Pulmonary Disease

Man Zhu*1

1Department of Laboratory Medicine, West China Hospital, Sichuan University

Radiomics and Clinical Features for Classifying Transcriptome-Defined Immune Subgroups in Non-Small Cell Lung Cancer

Liqun Huang1,

Yangbin Dai2,

Yingrui Su1,

Jianlin Wang1,

Long Xie*1

1Department of Nuclear Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China,

2Department of Oncology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China

Metabolic and Bone Formation Markers for Identifying Osteoporosis in Rheumatoid Arthritis in a Cross-Sectional Analysis

Lirong Chen1,

Zhihong Wu1,

Chao Jia1,

Huiqin Yang*1

1Department of Rheumatology and Immunology, Wuhan No.1 Hospital

Experimental Approaches to Assessing Aging Effects of Emerging Contaminants: Model Selection, Endpoints, and Interpretation

Xiaoling Li1,

Man Zhu*1

1West China Hospital, Sichuan University

Discordant Cardiac Troponin T and I: Mechanistic Insights, Analytical Interference, and Translational Diagnostic Strategies

Xiaoling Li1,

Man Zhu*1

1Department of Laboratory Medicine, West China Hospital, Sichuan University