

Man Zhu
Department of Laboratory Medicine, Sichuan University
Dr. Man Zhu is an assistant research fellow in the Department of Laboratory Medicine, West China Hospital, Sichuan University. He received his doctoral degree in medicine from the University of Electronic Science and Technology of China. His research focuses on aging intervention mechanisms, healthspan regulation, and aging-related diseases, with particular interest in brown adipose tissue dysfunction, skeletal muscle aging, sarcopenia, senescence-associated secretory phenotype, epigenetic remodeling, and topoisomerase-mediated molecular regulation.Dr. Zhu has served as the principal investigator for two research projects, including one supported by the Department of Science and Technology of Sichuan Province and one institutional project supported by West China Hospital. He has published more than 10 SCI-indexed articles, including representative studies published in PNAS, Aging Cell, and ACS Nano. By integrating clinical observations with animal models, cellular experiments, and molecular approaches, his work aims to elucidate key mechanisms of aging and identify potential strategies for delaying aging-related functional decline.

Xue Wen
Department of Plastic and Burn Surgery, Sichuan University
Dr. Xue Wen is an attending physician and postdoctoral fellow in the Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, Chengdu, China. She received her medical doctorate through the eight-year Clinical Medicine Program at Wuhan University. Her research focuses on environmental health, endocrine-disrupting chemicals, epigenetic regulation, adipose tissue dysfunction, and metabolic diseases.By integrating epidemiological studies, animal models, molecular biology techniques, and epigenomic analyses, Dr. Wen investigates how environmental exposures disrupt metabolic homeostasis and contribute to obesity and other chronic diseases. She has served as the principal investigator for one project funded by the National Natural Science Foundation of China, one provincial- or ministerial-level project, and one institutional project supported by West China Hospital. She has published in peer-reviewed international journals, including PNAS, Environmental Pollution, Journal of Molecular Endocrinology, and ACS Nano.
Aging is a central driver of functional decline across tissues and organs and is closely linked to a broad range of chronic conditions, including metabolic disorders, sarcopenia, neurodegenerative diseases, cardiovascular diseases, impaired wound healing, and frailty. As the burden of aging-related diseases continues to increase, there is a growing need for reliable methods that support earlier diagnosis, functional evaluation, and effective intervention. Despite rapid advances in aging research, the translation of laboratory findings into reproducible diagnostic and therapeutic approaches remains challenging because aging-related diseases involve complex changes in cellular senescence, inflammation, metabolic homeostasis, tissue remodeling, and systemic function.
This JoVE collection aims to present reproducible, visually demonstrable, and application-oriented protocols for diagnosis, functional evaluation, and intervention in aging and aging-related diseases. Topics of interest include molecular and imaging-based diagnostic approaches, biomarker detection, cellular senescence and senescence-associated secretory phenotype assays, organ and tissue functional assessment, aging-related animal and cellular models, biomaterials- or nanomaterials-based diagnostic and delivery platforms, and therapeutic strategies targeting metabolic, musculoskeletal, regenerative, or functional decline.
By providing step-by-step video protocols and detailed methodological guidance, this collection will help researchers and clinicians standardize experimental procedures, reduce technical variability, and improve the reproducibility and translational impact of aging research. It will serve as a practical resource for investigators developing diagnostic tools, disease models, and intervention strategies for aging biology and aging-related diseases.
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2026
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Jun-Ling Wang*1,2, Cai-E Hou*3, Kun-Rui Chen*4, Han-Min Wang5, Cheng-Yun Zhang6, Xiu-Ping Li1,2, Jian-Peng Hu1,2, Hong-Xia Liu1,2, Zhao-Min Chen2, Chang Wen7, Yin-Yu Zhou1, Jian-Mei Song1, Min-Fang Wu1, Min Chen1, Fan Zhang1, Shu-Min Li1, Ruo-Shui Kong1, Ying-Yu Yang8, Fei He2,9, Hongwei Li1,2,10,11
1Clinical Laboratory, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 2Yunnan Provincial Department of Education, Engineering Research Center for Precision Medical Testing, Kunming Medical University, Qujing First People's Hospital, 3Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 4Department of Infectious Diseases, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 5Department of Endocrinology and Metabolism, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 6Department of Respiratory Medicine, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 7Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, 8Department of Pathology, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 9Department of Orthopedics, Qujing University of Medicine and Health Sciences, Qujing First People's Hospital, 10Yunnan Key Laboratory of Laboratory Medicine, 11Yunnan Province Clinical Research Center for Laboratory Medicine
Prolonged Rewarming Was Associated with Reduced Alkaline Phosphatase Bias in an External Quality Assessment Sample: A Laboratory Study
Dayun Xie1,
Man Zhu*2
1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,
2Department of Laboratory Medicine, West China Hospital, Sichuan University
Hypercalcemia and Hypophosphatemia in a Patient with Gastrointestinal Symptoms: A Diagnostic Case of Primary Hyperparathyroidism
Dayun Xie1,
Man Zhu*2,
Jiao Liu*1
1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,
2Department of Laboratory Medicine, West China Hospital, Sichuan University
Persistent Hypokalemia and Hypomagnesemia Leading to the Diagnosis of Gitelman Syndrome: A Laboratory-Guided Case Report
Dayun Xie1,
Xue Wen2,
Man Zhu*3,
Jiao Liu*1
1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,
2Department of Plastic and Burn Surgery, West China Hospital, Sichuan University,
3Department of Laboratory Medicine, West China Hospital, Sichuan University
Multiwavelength Photometric Correction Reduces Lipemia-Associated Bias in Four Clinical Chemistry Assays: An In Vitro Interference Study
Dayun Xie1,
Wenbiao Zhou1,
Baoxiu Chen1,
Yali Li1,
Xue Wen*2,
Man Zhu*3,
Jiao Liu*1
1Department of Laboratory Medicine, Chuxiong Yi Autonomous Prefecture People's Hospital,
2Department of Plastic and Burn Surgery, West China Hospital, Sichuan University,
3Department of Laboratory Medicine, West China Hospital, Sichuan University
Early Nutritional Risk Assessment and Structured Nursing Care in Hemorrhagic Stroke: A Propensity-Matched Cohort Study
Haiyan Zhang1,
Yanzhen Shi*2
1Interventional Medical Center, The Second People's Hospital of Hunan Province (Brain Hospital of Hunan Province), Changsha, Hunan 410007, China,
2Department of Cardiovascular Medicine, The Second People's Hospital of Hunan Province (Brain Hospital of Hunan Province), No. 427, Section 3, Furong Middle Road, Changsha, Hunan 410007, China.
A Tandem Mass Tag-Based Quantitative Urinary Proteomic Workflow for Identifying Candidate Protein Markers in Patients with Gastric Cancer
Yadan Wang1
1Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases
Circulating NT-proBNP and soluble ST2 for Early Cardiac Dysfunction in Older Patients With Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Man Zhu*1
1Department of Laboratory Medicine, West China Hospital, Sichuan University
Radiomics and Clinical Features for Classifying Transcriptome-Defined Immune Subgroups in Non-Small Cell Lung Cancer
Liqun Huang1,
Yangbin Dai2,
Yingrui Su1,
Jianlin Wang1,
Long Xie*1
1Department of Nuclear Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China,
2Department of Oncology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
Metabolic and Bone Formation Markers for Identifying Osteoporosis in Rheumatoid Arthritis in a Cross-Sectional Analysis
Lirong Chen1,
Zhihong Wu1,
Chao Jia1,
Huiqin Yang*1
1Department of Rheumatology and Immunology, Wuhan No.1 Hospital
Experimental Approaches to Assessing Aging Effects of Emerging Contaminants: Model Selection, Endpoints, and Interpretation
Xiaoling Li1,
Man Zhu*1
1West China Hospital, Sichuan University
Discordant Cardiac Troponin T and I: Mechanistic Insights, Analytical Interference, and Translational Diagnostic Strategies
Xiaoling Li1,
Man Zhu*1
1Department of Laboratory Medicine, West China Hospital, Sichuan University