The Staphylococcus bacteria are opportunistic pathogens that exist as a part of the normal microbiota of human or animal skin and mucous membranes. There is remarkable global significance of Staphylococcus aureus infections because of increasing antibiotic resistance of bacteria and additional properties as different localization, manifestation, and course of the diseases. However, changes in patient populations, such as increased number of premature neonates, elderly and immunocompromised patients, and the increasing use of implanted foreign prosthetic materials and indwelling catheters have led to a rise in documented infections caused by coagulase-negative staphylococci (CoNS) and coagulase-positive staphylococci  (CoPS) other than S. aureus. The most emerging questions related to staphylococcal diseases are connected to transfer of mobile genetic elements leading to increased virulence and/or antibiotic resistance, the proper identification at the species level, and host-pathogen interactions often modulated with diverse regulatory systems. The proposed collection will bring together visualized experiments that demonstrate highly innovative and currently used approaches for the challenges of infections caused by Staphylococcus species. The presented methods will focus on: (i) proper microbiological material collection from various isolation sites, (ii) detection of antibiotic resistance, (iii) genetic methods used for species fast identification and characterization including those based on next-generation sequencing, (iv) targeted diagnostics, (v) broad aspects of cell genetic regulation in staphylococci, and (vi) bioinformatic tools used for data analysis.