Apolipoprotein Analysis
Apolipoprotein Analysis - Related Videos
10:59
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Cited by 32 •
2013
Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.
07:36
Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
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Cited by 8 •
2018
We describe a protocol to induce atherosclerosis in the aortic root of ApoE-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.
07:08
A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
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Cited by 3 •
2016
We describe a high-throughput, multiplex, and targeted proteomic cerebrospinal fluid (CSF) assay developed with potential for clinical translation. The test can quantitate potential markers and risk factors for neurodegeneration, such as the apolipoprotein E variants (E2, E3 and E4), and measure their allelic expression.
07:37
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
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Cited by 2 •
2020
Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.
10:39
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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Cited by 3 •
2022
Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
FAQs
Apolipoproteins provide the protein framework associated with lipid-carrying particles and participate in their transport and metabolic behavior. Their production, molecular modification, and clearance can change how lipoproteins are distributed in blood. Measuring these features therefore adds information about lipoprotein abnormalities that may not be apparent from cholesterol and triglyceride concentrations alone.
Cholesterol and triglyceride results describe major lipid contents, whereas apolipoprotein analysis examines associated proteins and their distribution among lipoproteins. This additional perspective can help distinguish abnormalities in lipoprotein structure or metabolism. In biochemistry and cardiovascular research, combining these measurements supports more detailed assessment of lipid-related disease processes and risk.
Immunochemical assays primarily quantify selected apolipoproteins, while electrophoresis, chromatography, and mass spectrometry provide information about distribution, interactions, or molecular composition. These approaches address different analytical questions rather than serving as interchangeable measurements. Their use can clarify how apolipoproteins associate with lipoproteins and how molecular differences relate to abnormal lipid metabolism.
Measured concentrations reflect more than synthesis alone. Production determines how much apolipoprotein enters the system, modification can alter its molecular characteristics or interactions, and clearance influences how long it remains detectable. Considering these processes helps biochemists interpret unusual results mechanistically and investigate whether an observed pattern reflects altered metabolism, molecular change, or removal.
Apolipoprotein assays can be performed on serum or plasma, with immunochemical methods used to quantify specific proteins. The analytical approach should match the intended result: concentration measurements require a suitable immunochemical assay, whereas distribution or molecular characterization may call for electrophoresis, chromatography, or mass spectrometry. This distinction guides both sample analysis and interpretation.
Researchers use these measurements to investigate lipoprotein abnormalities and disorders of lipid metabolism, especially when protein composition or particle distribution is important. The results can complement routine lipid testing and help characterize biochemical patterns associated with cardiovascular risk. They also support studies of how apolipoprotein production, modification, and clearance contribute to disease.
Apolipoprotein analysis contributes to research on atherosclerosis, metabolic disease, biomarker development, and therapeutic responses. Quantitative assays can track selected proteins, while broader analytical methods examine molecular composition and interactions. Together, these data help researchers evaluate candidate biomarkers, characterize disease-related lipid changes, and assess biochemical effects associated with treatment.
Within biochemistry, the topic connects protein measurement with lipid transport, lipoprotein organization, and metabolism. It enables investigators to examine not only how much of a protein is present, but also where it is distributed and how it interacts within lipid-carrying systems. This integrated view supports mechanistic studies of cardiovascular and metabolic disorders.