Bio-orthogonal Crosslinking
Bio-orthogonal Crosslinking - Related Videos
01:26
Orthogonal Trajectories
0 Views •
2026
Orthogonal trajectories describe the geometric relationship between two families of curves that intersect each other at right angles. One illustrative case involves a family of parabolas that open sideways along the x-axis. These curves share a common shape but differ by a scaling parameter, resulting in a set of curves that all pass through the origin and widen at different rates.Determining Orthogonal TrajectoriesTo identify the orthogonal trajectories for these parabolas, the first step...
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
0 Views •
2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
10:32
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
0 Views •
Cited by 7 •
2012
A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.
11:15
Nanosponge Tunability in Size and Crosslinking Density
0 Views •
Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
0 Views •
Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
FAQs
The key reaction pairs tetrazines with strained alkenes in a rapid, catalyst-free click process. Because these complementary groups react selectively in biological environments, crosslinking can proceed under mild, aqueous conditions rather than requiring conditions that disrupt native cellular chemistry. This selectivity supports formation of biomaterial networks in the presence of living cells.
Once formed, the network's properties can be tuned through the biomaterial components and their crosslinking behavior. The resulting structure may provide selected mechanical properties, degradation characteristics, and biochemical presentation. In bioengineering, these parameters matter because they influence how a material supports cells, presents signaling molecules, or releases therapeutic cargo.
Avoiding an added catalyst helps keep the reaction compatible with biological environments and native cellular chemistry. The tetrazine-strained alkene pairing can therefore be used under mild, aqueous conditions, where functionalized polymers or proteins are joined without introducing a catalyst-dependent crosslinking step. This feature is especially relevant when cells must remain present during material formation.
A basic workflow begins with functionalizing polymers or proteins with complementary reactive groups. The modified components are then brought together in a mild, aqueous environment, where the groups react and create a covalent network. Depending on the design, this process can be performed while cells are present, enabling fabrication of cell-containing biomaterials.
Injectable hydrogels are a major format because crosslinking can create the network after delivery at the intended site. The same strategy can immobilize signaling molecules, encapsulate cells, support controlled drug delivery, or contribute to tissue repair. These uses connect reaction selectivity with practical biomaterial functions in regenerative medicine and other biomedical applications.
The ability to crosslink in the presence of living cells enables minimally invasive biomaterial fabrication, a useful feature for regenerative medicine. Engineers can create cell-containing hydrogels and related materials while maintaining compatibility with cellular chemistry. This capability expands applications toward tissue repair, biochemical signaling, and controlled delivery while allowing the network's properties to be adjusted for biological needs.