Bladder Wall Anastomosis
Bladder Wall Anastomosis - Related Videos
03:48
Mouse Bladder Wall Injection
0 Views •
Cited by 30 •
2011
Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.
04:15
Bladder Tumor Organoid Model: A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder
0 Views •
2023
In this video, we describe the orthotopic transplantation of cultured bladder tumor organoids into the murine bladder to study the role of the organ environment on developing tumors.
10:19
Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
0 Views •
Cited by 20 •
2012
Surgical stages of bladder augmentation are described using 3-D scaffolds in murine and rat models. To test the efficacy of biomaterial configurations for use in bladder augmentation, techniques for both awake and anesthetized cystometry are presented.
04:28
Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder
0 Views •
2023
In this video, we describe the intravesical procedure of delivering cancer cells into the bladder of a mouse model. This procedure helps generate an orthotopic bladder tumor model that can be used to develop therapeutic strategies.
08:39
Culture of Bladder Cancer Organoids as Precision Medicine Tools
0 Views •
Cited by 4 •
2021
Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.
FAQs
Maintaining the wall’s layered organization helps the repaired region remain structurally continuous while it heals. The layers contribute to the integrity of the urinary reservoir, so disrupting their arrangement could compromise the repair’s ability to contain urine. This principle is especially important when joining native tissue segments or connecting native bladder tissue with an engineered construct.
Vascularization supplies the biological support needed for healing, while tissue integration allows the joined region to become more continuous with surrounding bladder wall. These processes extend the repair beyond immediate mechanical fixation. In bioengineering, they are central considerations because an engineered graft must form a functional interface with native tissue rather than remain an isolated implant.
The interface must connect two different sources of tissue: the patient’s native bladder wall and a biomaterial scaffold or tissue-engineered construct. Its quality influences whether the reconstructed region can become continuous with the surrounding wall and participate in urinary containment. Consequently, anastomosis serves as a critical design and repair consideration in engineered urinary tract reconstruction.
Several features work together: viable tissue edges provide material for healing, accurate alignment restores continuity, and sutures or other fixation methods maintain the connection. The repair must also form a watertight seal and preserve the wall’s layered structure. Together, these conditions support urine containment while the joined tissues undergo vascularization and integration.
The process begins by bringing viable bladder tissue edges, or native and engineered tissue interfaces, into appropriate alignment. Sutures or another fixation method then secure the connection and establish a watertight seal. The repaired region subsequently depends on healing, vascularization, and tissue integration to develop a durable, continuous bladder wall.
Bioengineering research applies this approach when bladder tissue has been injured, resected, or selected for reconstruction with a scaffold or tissue-engineered construct. It provides a practical interface for evaluating how engineered materials connect with native tissue. Outcomes of interest include restoration of a continuous urinary reservoir, reduced leakage, and support for regenerative urinary tract reconstruction.