Breast Organoids
Breast Organoids - Related Videos
08:29
Establishment and Culture of Patient-Derived Breast Organoids
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Cited by 11 •
2023
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
06:42
Optimization of Breast Biopsy and Mastectomy Sample Collection Procedures for Biobanking, Personalized Medicine, and Research Applications
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2025
This article presents a detailed protocol for collecting breast cancer samples, designed to optimize their utility for biobanking, personalized medicine, and other research applications. The workflow for sample collection, including breast biopsy and mastectomy procedures, is described in detail, focusing on minimizing tissue damage and maximizing sample quality.
07:13
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
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Cited by 24 •
2014
Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.
10:10
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
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Cited by 11 •
2011
We have developed a collagen-based in vitro assay which promotes proliferation and invasion from samples of all breast cancer subtypes. Optical Projection Tomography, a three dimensional microscopy technique was utilised to visualise and quantify tumour expansion. This assay may be used to quantify drug response of individual tumour samples.
07:45
Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
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Cited by 15 •
2020
Patient-derived xenograft (PDX) models and transplantable genetically engineered mouse models faithfully recapitulate human disease and are preferred models for basic and translational breast cancer research. Here, a method is described to orthotopically transplant breast tumor fragments into the mammary fat pad to study tumor biology and evaluate drug response.
FAQs
Breast organoids develop tissue-like organization through two interacting sources of information: signals exchanged between neighboring cells and cues supplied by the extracellular matrix. Together, these inputs influence whether mammary epithelial cells proliferate, differentiate, or assemble into particular structures. This interaction matters in bioengineering because the surrounding material is not merely a scaffold; it helps shape the cellular behavior being studied.
Compared with conventional two-dimensional cultures, these models preserve more of the spatial organization associated with breast tissue. Cells can interact with one another and with a surrounding matrix in three dimensions, allowing researchers to examine structural features alongside cellular behavior. The comparison is useful when a flat culture cannot adequately represent tissue organization or when compound responses may depend on that organization.
Outcomes depend on how mammary epithelial cells respond to cell-cell communication and matrix interactions. Those influences can alter proliferation, differentiation, and the formation of tissue-like structures, producing models of normal or diseased breast tissue. In practice, researchers must interpret the resulting organization and behavior together rather than treating cell growth alone as the complete experimental result.
A controllable culture platform allows researchers to investigate breast-tissue organization and function under defined laboratory conditions. This makes it possible to focus on how cellular signaling and matrix interactions relate to proliferation, differentiation, or disease-associated features. For bioengineering, that control supports systematic model development and helps connect engineered culture conditions with observable tissue-like outcomes.
A basic workflow begins by placing mammary epithelial cells within a supportive extracellular matrix material and maintaining the culture so that self-organization can occur. Researchers then examine the resulting tissue-like structures, functional features, or responses to therapeutic compounds. This sequence links the engineered environment to measurable changes in organization and behavior without reducing the experiment to a two-dimensional layer.
The central engineered components are mammary epithelial cells and a supportive extracellular matrix material. The matrix provides the context in which cell-cell signaling and cell-matrix interactions can guide growth and organization. Combining these components is important because the model's usefulness depends on reproducing relevant structural and functional features rather than simply sustaining cells in culture.
Applications span mammary development, tissue organization, disease mechanisms, and evaluation of therapeutic compounds. Because the cultures provide a controllable laboratory platform, researchers can investigate these questions within a breast-tissue-related context while varying the biological condition under study. The same framework also supports tissue engineering and the development of experimental models intended to improve on conventional culture systems.
Models representing normal or diseased breast tissue can help researchers examine how organization and functional behavior vary across conditions. Their responses to therapeutic compounds provide an experimental readout relevant to treatment research, while their controllable nature supports personalized research. These uses make the cultures valuable for focused laboratory investigations of breast biology and treatment-related responses.