Calcium Dependent Adhesion
Calcium Dependent Adhesion - Related Videos
08:51
Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
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Cited by 13 •
2012
This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.
03:22
Calcium-Dependent Hydrophobic Interaction Chromatography: A Technique to Purify Calcium-Binding Proteins Based on Hydrophobic Interactions
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2025
In this video, we demonstrate the purification of calcium-binding protein from a dialyzed cell lysate through calcium-dependent hydrophobic interaction chromatography. The calcium-binding proteins expose a hydrophobic region upon binding with calcium, facilitating interaction with a hydrophobic group on resin. Later these proteins are eluted using calcium chelator EDTA that reverses the interaction.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
08:24
Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
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Cited by 1 •
2021
This protocol presents the experimental procedures to perform the adhesion footprint assay to image the adhesion events during fast cell rolling adhesion.
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Cited by 30 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
FAQs
Calcium binding to extracellular cadherin domains stabilizes the protein conformation needed for contacts between cadherins on neighboring cells. These contacts are selective because cadherins favor homophilic pairing, meaning the same type of adhesion molecule interacts across the cell boundary. When calcium is removed, that stabilization is lost and cell-cell attachment can weaken or break.
Calcium-dependent control links individual molecular contacts to larger-scale tissue behavior. Stabilized cadherin interactions help neighboring cells remain organized into cohesive arrangements, whereas weakened contacts can permit loss of cohesion and remodeling. This makes calcium availability relevant not only to whether cells attach, but also to how tissue-like structures maintain or change their organization.
Altering calcium availability provides a way to modulate the state of cadherin-based contacts. Conditions that support calcium binding favor stabilized interactions, while calcium removal weakens or disrupts them. In engineered systems, this control can be used to adjust cell attachment, mechanical cohesion, and remodeling, allowing adhesion behavior to be matched to the intended tissue or material design.
Here, adhesion strength and continuity are linked to the presence of calcium at extracellular cadherin domains. That dependence creates a controllable interaction: maintaining calcium supports cadherin conformation and neighboring-cell contacts, whereas removing it destabilizes the adhesive state. The distinction matters in bioengineering because calcium can serve as a design variable rather than only a background condition.
A bioengineered surface can be designed to support cell attachment through the same calcium-regulated cadherin interactions that organize neighboring cells. The relevant design goal is not simply adhesion, but adjustable attachment and cohesion. Controlling calcium availability in the system can therefore help tune how cells engage the surface and how the resulting cell-associated structure remodels.
In engineered tissues and organoids, cadherin-mediated contacts provide a molecular basis for cells to assemble into organized, cohesive structures. Calcium control can influence whether those contacts remain stabilized or become weaker, affecting attachment and remodeling during tissue construction. Consequently, the mechanism helps bioengineers model tissue assembly while retaining a means to adjust structural cohesion.
Biomimetic materials can use calcium-dependent adhesion as a model for reproducing a key feature of tissue organization: selective cell-cell attachment that can be strengthened or weakened through molecular stabilization. This approach connects material design with biological behavior, allowing researchers to consider cell attachment, mechanical cohesion, and remodeling together rather than treating adhesion as a fixed property.