Cardiac Progenitors
Cardiac Progenitors - Related Videos
07:02
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
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Cited by 9 •
2022
We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.
08:00
Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
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Cited by 6 •
2015
In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
10:03
Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
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Cited by 5 •
2023
Here, we present a protocol describing cell nuclei preparation. After microdissection and enzymatic dissociation of cardiac tissue into single cells, the progenitor cells were frozen, followed by isolation of pure viable cells, which were used for single-nucleus RNA sequencing and the single-nucleus assay for transposase-accessible chromatin with high-throughput sequencing analyses.
05:04
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
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Cited by 4 •
2022
We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca2+) flux.
FAQs
Developmental signals act as control inputs that influence cardiac progenitor behavior. In engineered studies, researchers use these signals to examine how cells maintain self-renewal, commit to a lineage, or differentiate into cardiomyocytes, endothelial cells, and smooth muscle cells. This makes signaling central to connecting developmental biology with the design of culture and tissue models.
The balance between self-renewal, lineage commitment, and differentiation determines how a cardiac progenitor population develops. Maintaining self-renewal preserves an early-stage cell pool, whereas commitment directs cells toward specialized heart cell types. Studying this balance helps bioengineers investigate how developmental regulation affects the cellular composition and formation of engineered cardiac tissues.
Generating cardiomyocytes, endothelial cells, and smooth muscle cells provides a broader cellular basis for studying heart development and tissue formation. Models containing these differentiated populations can represent cardiac organization more meaningfully than systems focused on a single cell type. This cellular range also supports investigations of repair and the construction of engineered heart tissues.
Researchers combine cardiac progenitors with biomaterials, engineered culture systems, or three-dimensional tissue models. These platforms provide structured experimental settings for examining development, cell organization, and tissue formation. The resulting models can be used to investigate how progenitor behavior contributes to more physiologically relevant cardiac systems, while also exploring conditions that may improve cell survival and integration.
Biomaterials and engineered culture systems provide the surrounding framework for studying cardiac progenitor behavior outside conventional experimental settings. When paired with these cells, they support investigations of organization, tissue formation, and developmental processes. Their value in bioengineering comes from enabling models that may more closely reflect cardiac physiology than simpler culture arrangements.
Cardiac progenitors are useful when researchers need engineered cardiac models for studying heart development, disease-related processes, or responses relevant to drug evaluation. Three-dimensional systems can provide more physiologically relevant platforms for these investigations. Such models also connect cellular differentiation and tissue organization with practical questions about cardiac function and therapeutic testing.