Collagen Flow Rate
Collagen Flow Rate - Related Videos
13:35
Mass Conservation and Flow Rate Measurements
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The purpose of this experiment is to demonstrate the calibration of a flow passage as a flowmeter using a control volume (CV) formulation [1, 2]. The CV analysis focuses on the macroscopic effect of flow on engineering systems, rather than the detailed description that could be achieved with a detailed differential analysis. These two techniques should be...
04:30
A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow
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Cited by 2 •
2024
We present a method for replicating the glioma tumor microenvironment at the invasive front that incorporates interstitial fluid flow. This model is a hyaluronan-collagen I hydrogel in a tissue culture insert where a fluid pressure head can be applied. Invasion can be quantified, and cells can be isolated or lysed.
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
07:12
Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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Cited by 3 •
2022
This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.
07:03
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Cited by 18 •
2014
This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.
FAQs
Viscosity affects how readily the collagen solution moves through the device and therefore changes the relationship between pump settings, applied pressure, and delivered material. A more viscous solution can alter shear conditions and filament formation compared with a less viscous one. Accounting for this variation helps maintain consistent collagen distribution across experiments.
Applied pressure and pump settings directly influence how much collagen solution reaches the outlet over a given period. If these controls are not coordinated, material delivery can vary even when the device configuration remains unchanged. Monitoring their effect supports reproducible deposition and helps researchers compare fabrication conditions without confusing control settings with biological or structural outcomes.
Nozzle geometry determines how the collagen solution is directed through the device and contributes to the shear conditions experienced during extrusion. Because flow rate interacts with this geometry, changing the nozzle can alter filament formation and material distribution. Researchers therefore need to interpret flow measurements together with the dispensing configuration when optimizing collagen-based structures.
Flow conditions influence the mechanical stress imposed on collagen solutions and any cells carried within them. Excessive stress may compromise the intended cell-compatible fabrication environment, while poorly controlled delivery can produce uneven material placement. Tuning the rate helps balance consistent collagen deposition with conditions that reduce unnecessary mechanical loading during construct fabrication.
A practical setup considers solution viscosity, applied pressure, pump settings, and nozzle geometry together rather than selecting a rate in isolation. Researchers can then observe how those variables affect filament formation, collagen distribution, and the timing of deposition or gelation. This coordinated evaluation provides a basis for choosing conditions that produce repeatable scaffold structures.
Recording and controlling the rate provides a common process parameter for evaluating collagen-based prints and scaffolds. Differences in viscosity, pressure, pump operation, or nozzle design can otherwise change material delivery and structural outcomes. Linking measured flow behavior with filament formation and deposition timing makes results easier to interpret across experimental systems and fabrication runs.