Core Cladding Flow

Core cladding flow is a fluidic configuration in which a central core stream travels within a surrounding cladding stream, enabling precise control of interfaces and material organization. In microfluidic devices, separately introduced fluids move in parallel under predominantly laminar conditions, allowing the cladding phase to focus, sheath, or encapsulate the core without turbulent mixing. This arrangement supports the formation of uniform droplets, fibers, particles, and cell-containing structures with controlled dimensions and composition. In bioengineering, core cladding flow is therefore useful for biomaterial fabrication, cell encapsulation, tissue engineering, and the development of microscale delivery systems.

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Education

JoVE Core - Molecular Biology

The Nucleosome Core Particle

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2020

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...

Research

JoVE Journal - Medicine

Hyperglycemic Clamp and Hypoglycemic Clamp in Conscious Mice

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Cited by 4 •

2024

A hyperglycemic clamp is used for measuring insulin release with a maintained higher blood glucose concentration. A hypoglycemic clamp is for measuring glucose production induced by counter-regulatory responses. Both methods use the same surgical procedure. Here, we present a clamp technique to assess systemic glucose metabolism.

Sediment Core Sectioning and Extraction of Pore Waters under Anoxic Conditions

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Cited by 5 •

2016

A protocol for sectioning sediment cores and extracting pore waters under anoxic conditions in order to permit analysis of redox sensitive species in both solids and fluids is presented.

Patch Clamp Electrophysiology

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2023

Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.

Research

JoVE Journal - Environment
Free Sample

A New Workflow for Sampling and Digitizing Increment Cores

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Cited by 11 •

2024

We present a protocol to use 3D-printed mounts to fix increment cores in the field without the necessity of unpacking and gluing them on wooden mounts. The new GSC holder allows placing the cores in a core microtome to cut their surface and directly transfer them to digital image capturing.

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