Electrochemical Detection
Electrochemical Detection - Related Videos
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
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Cited by 6 •
2013
We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
07:39
Electrochemical Biosensing
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2023
Electrochemical biosensors detect the binding of a target molecule by sensing an oxidation-reduction event. These sensors paved the way for modern biosensing after the invention of the glucose biosensor. This video will introduce electrochemical biosensing, show the workings of the glucose biosensor, and discuss how electrochemical biosensors are used in cancer detection.
12:11
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
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Cited by 7 •
2009
A protocol to detect trichothecenes (mycotoxins of concern for human health) using a newly developed screening method based on a competitive immunochemical method and a final electrochemical detection is demonstrated.
13:15
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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Cited by 21 •
2011
"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.
01:26
What is an Electrochemical Gradient?
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2026
Adenosine triphosphate, or ATP, is considered the primary energy source in cells. However, energy can also be stored in the electrochemical gradient of an ion across the plasma membrane, which is determined by two factors: its chemical and electrical gradients.The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an ion’s...
FAQs
The signal type depends on which electrical consequence of the analyte interaction is monitored. Oxidation or reduction can generate a measurable current, while changes in electron transfer at the electrode can appear as shifts in potential or impedance. Selecting among these readouts allows a sensor design to match the chemical or biological event being measured and its concentration-related response.
Concentration is inferred from how strongly the measured electrical quantity changes as analyte amount changes. This relationship supports both detection, determining whether a substance is present, and quantification, estimating how much is present. In bioengineering, that link lets one electrical measurement represent levels of metabolites, nucleic acids, proteins, or pathogens in a sample.
The electrode surface is the point where the analyte's chemical activity becomes an electrical signal. Oxidation, reduction, or altered electron transfer at that interface changes the measured response. Because the surface event connects an analyte reaction with current, potential, or impedance, electrode behavior is central to obtaining a concentration-related measurement from biological material.
A basic measurement sequence begins with bringing a sample containing the target substance to an electrode, allowing the analyte to undergo or influence electron transfer, and recording the resulting electrical response. The measured current, potential, or impedance is then related to analyte concentration. This workflow can be applied to chemical substances as well as biological targets in complex samples.
Bioengineering applications can target metabolites, nucleic acids, proteins, and pathogens, giving the platform broad coverage across chemical and biological analysis. The relevant target is the substance whose oxidation, reduction, or influence on electron transfer changes the electrode signal. This makes the same general sensing principle adaptable to molecular measurements and pathogen detection in complex samples.
Miniaturization makes these platforms useful when measurements must be rapid or require only a small sample volume. That combination supports point-of-care testing and wearable devices, where compact sensing can be positioned close to the user or sampling site. The same advantages also contribute to clinical diagnostics and environmental monitoring, extending electrochemical detection beyond laboratory-scale analysis.