Exosome Cargo Analysis
Exosome Cargo Analysis - Related Videos
06:08
Formulating and Characterizing an Exosome-based Dopamine Carrier System
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Cited by 6 •
2022
Here, we aimed to obtain a formulation by dopamine loading of the isolated exosomes of stem cells from Wharton's jelly mesenchymal stem cells. Exosome isolation and characterization, drug loading into the resulting exosomes, and the cytotoxic activity of the developed formulation are described in this protocol.
11:09
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
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Cited by 8 •
2014
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
11:02
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
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Cited by 43 •
2015
Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
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2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
09:43
Isolation and Characterization of RNA-Containing Exosomes
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Cited by 515 •
2012
This paper demonstrates methods for the isolation, purification and detection of exosomes, as well as techniques for analysis of their molecular content. These methods are adaptable for exosome isolation from both cell culture media and biological fluids, and can beyond analysis of molecular content also be useful in functional studies.
FAQs
The cargo profile provides a molecular basis for vesicle-mediated communication. Examining proteins, lipids, nucleic acids, and other contents together can reveal how one cell may influence tissue behavior, disease processes, or immune responses. In bioengineering, these profiles support comparison of native vesicles and help evaluate whether engineered exosomes carry components relevant to a desired biological effect.
Identity and purity checks establish that the measured molecules come from the intended exosome preparation. This foundation is important when comparing samples from biological fluids or cell cultures, because an inadequately characterized preparation can weaken conclusions about signaling or biomarkers. The checks also support reliable evaluation of engineered vesicles and their intended cargo.
These approaches provide complementary ways to characterize molecular contents. Sequencing, mass spectrometry, and targeted molecular assays can be selected according to the cargo or question being investigated, while together they broaden characterization across nucleic acids, proteins, lipids, and other components. Their results help build cargo profiles for biological interpretation or engineering decisions.
Cargo analysis examines whether engineered exosomes contain the intended molecular material, retain it over time, and produce relevant functional effects. Assessing loading addresses the amount or presence of cargo introduced, while stability indicates whether that cargo remains associated with the vesicles. Functional evaluation connects the molecular design to its effects in bioengineering applications.
A workflow begins by isolating exosomes from a biological fluid or cell culture. Researchers then verify vesicle identity and purity before characterizing the cargo with sequencing, mass spectrometry, or targeted molecular assays. Organizing the process in this order helps ensure that subsequent cargo measurements are interpreted from a defined preparation rather than from unverified material.
The approach is useful when researchers need to connect vesicle contents with tissue behavior, disease processes, or immune responses, or when they are developing engineered delivery systems. It supports biomarker development, therapeutic delivery, and regenerative medicine. Cargo measurements also provide a way to assess whether engineered exosomes achieve the intended loading, stability, and functional outcome.