Free Spectral Range

Free spectral range (FSR) is the frequency or wavelength spacing between adjacent resonant peaks of an optical cavity or spectrometer, indicating how often its transmission spectrum repeats. In a resonator, light undergoes repeated round trips, and constructive interference occurs when the optical path length supports an integer number of wavelengths; for a linear cavity, the frequency spacing is approximately c/(2nL), where n is refractive index and L is cavity length. In bioengineering, FSR guides the design and interpretation of fiber-optic sensors, interferometers, and resonant biosensors, helping researchers distinguish spectral features, select measurement ranges, and detect changes in refractive index or biomolecular binding.

Free Spectral Range - Related Videos

Education

JoVE Core - Statistics

Range

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2023

The range is one of the measures of variation. It can be defined as the difference between a dataset's highest and lowest values. For example, in the study of seven 16-ounce soda cans, the filled volume of soda was measured, thus producing the following amount (in ounces) of soda: 15.9; 16.1; 15.2; 14.8; 15.8; 15.9; 16.0; 15.5 Measurements of the amount of soda in a 16-ounce can vary since different subjects record these measurements or since the exact amount - 16 ounces of liquid, was not...

Research

JoVE Journal - Chemistry
Free Sample

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

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Cited by 1 •

2021

This protocol introduces Franck-Condon Lineshape Analyses (FCLSA) of emission spectra and serves as a tutorial for the use of ARL Spectral Fitting software. The open-source software provides an easy and intuitive way to perform advanced analysis of emission spectra including excited state energy calculations, CIE color coordinate determination, and FCLSA.

Using Spectral Reflectometry to Determine Myelinated Axon Diameters in a Fixed Mouse Brain Slice

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2025

Source: Kwon, J., et al., Spectral Reflectometric Microscopy on Myelinated Axons In Situ. J. Vis. Exp. (2018) This video demonstrates the use of spectral reflectometry with a hyperspectral confocal microscope to analyze interference patterns and determine myelinated axon diameters in fixed brain tissue slices.

¹H NMR: Long-Range Coupling

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2024

The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling. In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene π orbitals.

Research

JoVE Journal - Environment
Free Sample

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

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Cited by 31 •

2017

An experimental protocol is presented for assessment of soil grown plant root systems with RGB and hyperspectral imaging. Combination of RGB image time series with chemometric information from hyperspectral scans optimizes insights into plant root dynamics.

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