High-throughput Automation

High-throughput automation is the use of automated systems to perform, coordinate, and analyze large numbers of experimental or manufacturing operations with consistent timing and handling. In bioengineering, robotic liquid handlers, plate-based workflows, sensors, and software transfer samples between steps, while standardized protocols and data capture reduce manual variation and accelerate processing. These systems support rapid screening of biomolecules, cells, materials, and process conditions, enabling researchers to compare many experimental variables in parallel. By increasing reproducibility and experimental scale, high-throughput automation helps advance drug discovery, synthetic biology, diagnostics, tissue engineering, and bioprocess development.

High-throughput Automation - Related Videos

Research

JoVE Journal - Bioengineering

High-Throughput Optogenetics Experiments in Yeast Using the Automated Platform Lustro

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Cited by 3 •

2023

This protocol outlines the steps for utilizing the automated platform Lustro to perform high-throughput characterization of optogenetic systems in yeast.

Research

JoVE Journal - Biology
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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

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Cited by 6 •

2012

Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

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Cited by 46 •

2016

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

Research

JoVE Journal - Neuroscience
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Automated High-throughput Behavioral Analyses in Zebrafish Larvae

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Cited by 29 •

2013

Our laboratory developed a novel high-throughput automated imaging system that is useful for the detection of several different behaviors in 7-day-old zebrafish larvae. The system can be used for detecting subtle changes in behavior after the larvae have been exposed to environmental toxicants or pharmaceuticals.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 14 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

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