High-throughput Production

High-throughput production is a manufacturing approach that generates and processes many biological or engineered products in parallel, enabling rapid evaluation and scale-up in bioengineering. It combines miniaturized workflows, automation, standardized operating conditions, and rapid analytical screening to increase experimental capacity while maintaining consistent measurements. Researchers use high-throughput production to optimize biomaterial formulations, engineer cells or microorganisms, evaluate bioprocess parameters, and identify promising candidates for further development. By reducing material use and shortening iteration cycles, this approach supports more reproducible research, accelerates biomanufacturing development, and helps translate laboratory innovations into scalable technologies.

High-throughput Production - Related Videos

Research

JoVE Journal - Bioengineering
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Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

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Cited by 12 •

2018

A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 14 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Research

JoVE Journal - Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

Research

JoVE Journal - Biology
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High-throughput Saccharification Assay for Lignocellulosic Materials

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Cited by 14 •

2011

A simple, rapid method for determining the saccharification potential of large numbers of plant biomass samples is described. The automated platform for this analysis involves the preparation of the plant biomass for analysis in 96 well plates and the subsequent performance of pretreatment, hydrolysis and quantification of the sugars released.

Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Cited by 4 •

2022

Optogenetic control of microbial metabolism offers flexible dynamic control over fermentation processes. The protocol here shows how to set up blue light-regulated fermentations for chemical and protein production at different volumetric scales.

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