Image-guided Laser Degradation

Image-guided laser degradation is a bioengineering technique that uses real-time imaging to direct laser energy toward selected biological tissues or engineered materials, enabling localized removal or breakdown while limiting effects on surrounding regions. The imaging system identifies the target and helps control laser position, exposure, and dose; absorbed light then produces localized thermal, photochemical, or mechanical effects that degrade the chosen material. This approach supports precise microsurgery, tissue remodeling, device fabrication, and the study of structure-function relationships in living systems. By combining spatial selectivity with visual feedback, it can improve experimental control and advance minimally invasive therapeutic and engineering strategies.

Image-guided Laser Degradation - Related Videos

Research

JoVE Journal - Bioengineering
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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

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Cited by 10 •

2017

This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.

Research

JoVE Journal - Neuroscience

Laser-guided Neuronal Tracing In Brain Explants

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2015

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

Research

JoVE Journal - Neuroscience
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Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

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Cited by 3 •

2017

We describe the use of laser capture microdissection to obtain samples of distinct cell populations from different brain regions for gene and microRNA analysis. This technique allows the study of differential effects of traumatic brain injury in specific regions of the rat brain.

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

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Cited by 5 •

2022

This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes.

Research

JoVE Journal - Bioengineering

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

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Cited by 2 •

2022

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.

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