In Situ Imaging
In Situ Imaging - Related Videos
07:43
Analyzing Situations in Helping Behavior
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2023
Source: Julian Wills & Jay Van Bavel—New York University Social psychologists and personality psychologists both attempt to predict human behavior, but they focus on very different factors. Whereas personality psychologists focus on how personality traits, character, and individual differences affect behavior, social psychologists focus primarily on the power of social situations in shaping behavior. We often underestimate the role that social situations can play in determining behavior. We...
01:18
Types of Buying Situations
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2025
The concept of a buying situation is central to understanding consumer behavior in marketing. A buying situation refers to the specific context in which a consumer is making a purchasing decision. It encompasses factors such as the level of buyer involvement, the complexity of the decision, and the buyer's familiarity with the product or service. There are typically three categories of buying situations: new-task buying, modified rebuy, and straight rebuy. New-Task Buying occurs when a consumer...
07:36
Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects
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Cited by 6 •
2018
The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 35 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 56 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
FAQs
Preserving the original arrangement lets researchers interpret cells, molecules, and engineered materials in relation to neighboring structures and interfaces. Extraction or isolation can remove those relationships, making it harder to connect location with behavior. In situ observations therefore help reveal how an engineered microenvironment or construct organizes biological activity and how nearby components relate to one another.
Optical contrast can make structures visible without specifying a molecular target, whereas fluorescent probes, genetically encoded reporters, and other labels provide signals tied to selected cells, molecules, or activities. Microscopy then records those signals in the intact sample. The chosen signal determines which feature or activity can be visualized and linked to its surrounding context.
Following cells or materials over time can show changes that a single observation would miss. In living tissues and developing constructs, sequential imaging can document cell behavior, tissue organization, or material degradation as these processes unfold. This temporal record helps connect evolving structural patterns with biological function rather than treating each state as an isolated endpoint.
A practical workflow begins by keeping the sample, tissue, or construct intact, then selecting an appropriate optical contrast method, fluorescent probe, genetically encoded reporter, or other label. Microscopy detects the resulting signal, while the recorded images are interpreted for spatial relationships and changes over time. This process links image acquisition to evaluation of an engineered system.
Bioengineers can apply the approach to several readouts, including cell behavior within a construct, degradation of a biomaterial, organization of engineered tissue, and interactions at material or device interfaces. These observations place performance in its local context, allowing researchers to assess whether a designed microenvironment produces the intended biological arrangement or activity.
By retaining location and timing information, results can connect a design feature with a biological response. Imaging may show how an engineered microenvironment relates to cell behavior, tissue organization, or interface interactions. Such evidence supports evaluation and refinement of biomaterials and devices because researchers can relate structural changes within the construct to its observed biological function.