Janus Base Nanotubes
Janus Base Nanotubes - Related Videos
07:14
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Cited by 20 •
2020
The goal of this protocol is to show the assembly of a biomimetic nanomatrix (NM) with Janus base nanotubes (JBNTs) and fibronectin (FN). When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion.
08:55
Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
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Cited by 13 •
2022
This protocol describes the assembly of a layer-by-layer Janus base nano-matrix (JBNm) scaffold by adding Janus base nanotubes (JBNts), matrilin-3, and Transforming Growth Factor Beta-1 (TGF-β1) sequentially. The JBNm was fabricated and characterized; additionally, it displayed excellent bioactivity, encouraging cell functions such as adhesion, proliferation, and differentiation.
09:09
Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
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Cited by 3 •
2016
We present a protocol to synthesize Janus microhydrogels composed entirely of the same base material, poly(N-isopropylacrylamide) (PNIPAAm), with a clearly compartmentalized structure base on the phase separation of a supersaturated NIPAAm monomer solution. The synthesized Janus microhydrogels show unique properties such as anisotropic thermo-responsiveness and organophilic/hydrophilic loading capability.
06:26
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
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Cited by 41 •
2017
Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro.
09:55
Preparation of Janus Particles and Alternating Current Electrokinetic Measurements with a Rapidly Fabricated Indium Tin Oxide Electrode Array
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Cited by 2 •
2017
In this article, a simple method to prepare partially or fully coated metallic particles and to perform AC electrokinetic property measurements with a rapidly fabricated indium tin oxide (ITO) electrode array is demonstrated.
FAQs
Complementary hydrogen bonding and base stacking organize the molecular building blocks into an elongated arrangement. These noncovalent interactions collectively stabilize the association of neighboring units, allowing a hollow tubular architecture to emerge rather than an unstructured aggregate. In bioengineering, this cooperative assembly is important because it links molecular recognition between building blocks with the formation of a functional nanoscale structure.
The two differentiated faces create chemical asymmetry across the nanotube surface. That asymmetry can provide distinct interaction sites for molecular recognition, cargo binding, or contact with biological interfaces. Instead of presenting a uniform exterior, the structure offers chemically differentiated regions whose arrangement may help connect the nanotube's architecture with selective behavior in bioengineering systems.
Formation occurs under suitable solution conditions that allow hydrogen bonding, base stacking, and other noncovalent interactions to act together. If those interactions do not organize the molecular units effectively, the desired elongated hollow architecture may not develop. Controlling the assembly environment therefore matters for obtaining the intended nanotube structure and its associated binding or recognition properties.
A conceptual workflow begins with molecular building blocks that contain complementary recognition features and chemically distinct faces. The units are placed under solution conditions that support their noncovalent association, where hydrogen bonding and base stacking guide organization into elongated hollow tubes. The resulting architecture can then be considered for cargo binding, molecular recognition, or interaction with biological interfaces.
Their chemically differentiated surfaces can support selective molecular recognition and cargo binding, while the internal cavity provides a nanoscale space associated with the tubular architecture. These features make the platform relevant to biosensors, where selective interactions are useful, and to targeted delivery systems, where binding and biological-interface interactions may help connect the nanostructure with a desired bioengineering function.
They provide a model for studying programmable biomolecular materials whose structure arises from designed noncovalent interactions. Their combination of nanoscale dimensions, hollow interiors, and chemically distinct surfaces connects molecular self-assembly with engineered biological functions. This makes them useful for investigating how recognition, cargo association, and biological-interface interactions can be integrated into engineered nanostructures.