Microphysiological System
Microphysiological System - Related Videos
11:06
Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
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Cited by 12 •
2022
Drug-induced liver injury (DILI) is a major cause of drug failure. A protocol has been developed to accurately predict the DILI liability of a compound using a liver microphysiological system (MPS). The liver model uses the coculture of primary hepatic cells and translationally relevant endpoints to assess cellular responses to treatment.
08:29
A Cardiac Microphysiological System for Studying Ca2+ Propagation via Non-genetic Optical Stimulation
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Cited by 4 •
2025
Using light to control cardiac cells and tissue enables non-contact stimulation, thereby preserving the natural state and function of the cells, making it a valuable approach for both basic research and therapeutic applications.
06:20
Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems
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Cited by 3 •
2024
This protocol describes a reconfigurable membrane-based cell culture platform that integrates the open-well format with fluid flow capabilities. This platform is compatible with standard protocols and allows for reversible transitions between open-well and microfluidic culture modes, accommodating the needs of both engineering and bioscience laboratories.
07:06
Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule
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Cited by 3 •
2025
We present an optimized protocol for processing whole human kidneys to isolate and culture primary renal proximal tubule epithelial cells and the application of these cells in a three-dimensional, microfluidic, microphysiological platform to recapitulate the renal proximal tubule.
08:59
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
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Cited by 5 •
2020
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
FAQs
Microphysiological systems gain experimental relevance by exposing cells to controlled physiological cues rather than leaving them in a static environment. Fluid flow, mechanical forces, chemical gradients, and tissue-specific interactions can influence how cells behave within the engineered setting. Applying these inputs in defined combinations helps investigators examine human tissue responses under conditions that more closely reflect selected aspects of physiology.
Three-dimensional organization places living human cells or tissues within a structured environment, while biomaterials help form that engineered setting. Microfluidic platforms provide controlled spaces for fluid movement and other environmental inputs. Together, these components allow researchers to coordinate cellular arrangement with physical and chemical conditions, supporting more defined studies of organ functions and organ interfaces.
Perfusion provides a way to apply fluid flow through the engineered model and maintain controlled physiological conditions. This is important because fluid movement can act as a physiological cue and can be combined with mechanical forces, chemical gradients, or tissue-specific interactions. As a result, investigators can study tissue behavior under defined dynamic conditions rather than relying only on static culture environments.
Conventional cell cultures may not reproduce the combination of three-dimensional organization, fluid flow, mechanical forces, chemical gradients, and tissue-specific interactions present in human physiology. A Microphysiological System integrates selected cues within a controlled engineered environment, potentially capturing tissue behaviors that simpler cultures miss. This added physiological context can improve the relevance of disease and drug-related experiments.
A typical setup combines living human cells or tissues with biomaterials and a microfluidic platform. Researchers organize the biological material in a controlled three-dimensional environment and may incorporate perfusion to apply fluid flow. They can then impose additional physiological cues, including mechanical forces or chemical gradients, depending on the organ function or organ interface being investigated.
These systems are useful when investigators need to examine disease processes, drug efficacy, or drug toxicity under controlled human-relevant conditions. The engineered environment allows researchers to observe tissue behavior while applying defined physiological cues and interactions. Such studies can reveal responses that conventional cell cultures may not capture and can support more informative evaluation of candidate treatments.
In bioengineering, Microphysiological Systems provide a platform for designing controlled models of human tissue behavior and organ interfaces. Their use in disease modeling and drug efficacy or toxicity testing can improve experimental relevance by incorporating selected aspects of human physiology. Because they are engineered in vitro models, they may also help reduce reliance on animal models for some research questions.