Micropipette Manipulation
Micropipette Manipulation - Related Videos
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
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Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 32 •
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Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
07:02
Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing
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Cited by 1 •
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The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.
02:44
Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models
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Cited by 6 •
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Here, we describe micropipette-guided drug administration (MDA) as an alternative method to oral gavage that incentivizes the research animal to ingest treatments readily with minimal stress and discomfort.
07:39
Mouse In Vivo Placental Targeted CRISPR Manipulation
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Cited by 5 •
2023
Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.
FAQs
Pressure differences across the pipette regulate whether fluid enters, leaves, or remains within the pipette. The operator combines this pressure control with precise pipette movement to aspirate material, transfer it, or deliver contents into a selected target. This coordinated control supports localized handling while limiting unnecessary fluid movement around cells or other biological materials.
Pipette geometry and tip size influence the forces applied during contact with a cell or other small biological material. These features therefore affect how fluid moves and how force is transmitted to the target. Selecting an appropriate geometry helps researchers balance controlled aspiration, injection, positioning, or mechanical handling with the goal of minimizing disruption.
Microscopic observation lets the operator coordinate pipette motion, pressure changes, and contact with the target at microscale dimensions. Visual control is especially important when a cell must be positioned, paired, aspirated, or injected at a specific location. In bioengineering workflows, this feedback helps make localized manipulation more controlled and reproducible.
A typical workflow places the pipette and biological target under microscopic observation, brings the pipette into controlled contact, and adjusts pressure differences to aspirate, transfer, inject, or reposition the material. Pipette movement and contact are then coordinated according to the task. The workflow can be adapted for individual cells, embryos, organoids, or tissue-assembly steps.
Micropipettes may be made from fine glass or polymer, with the choice of pipette geometry and tip size affecting manipulation forces. The procedure also depends on controlled movement and pressure regulation while the target remains under a microscope. Together, these features provide the physical control needed for handling cells and other small biological materials.
Bioengineers use the technique for single-cell handling, embryo and organoid manipulation, microinjection, cell pairing, and assembly of engineered tissues. It can also support studies of cell mechanics and localized delivery of biomolecules. Because the method provides precise, minimally disruptive control, it helps researchers create reproducible microscale workflows and examine responses at the level of individual cells.