Mid-infrared Imaging
Mid-infrared Imaging - Related Videos
09:39
In-situ Tapering of Chalcogenide Fiber for Mid-infrared Supercontinuum Generation
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Cited by 5 •
2013
We describe a method for in-situ tapering of As2S3 fibers to achieve efficient mid-infrared supercontinuum generation. By tapering while monitoring the supercontinuum’s spectrum, the spectral width can be maximized for a fiber taper. In-situ fiber tapering can be applied to optimize the performance of other fiber-based devices.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
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2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
01:45
Visualization of Mouse Cerebral Thrombi Using Near-Infrared Fluorescence Imaging
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2025
Source: Kim, D. E., et al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates near-infrared fluorescent (NIRF) imaging to visualize thrombi in excised brain tissue labeled with a fibrin-specific probe. Near-infrared light excites the probe, causing the thrombus to fluoresce, enabling high-contrast thrombi visualization in the major cerebral artery and small cortical blood vessels.
01:31
Ed through Mid-point Method
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2024
At point A, the price is $6 for 120 units. Moving to point B, the price increases to $9, resulting in a decrease in quantity demanded to 80 units. This translates to a 50 percent increase in price and a 33.33 percent decrease in quantity, leading to a price elasticity value of 33.33/50 or 0.67. Conversely, moving from point B to A, entails a 33.33 percent decline in price and a 50 percent increase in quantity. This yields a price elasticity of 50/33.33 or 1.5. This discrepancy arises from the...
13:10
Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
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Cited by 8 •
2016
This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.
FAQs
The key signal comes from wavelength-specific absorption: molecular bonds absorb selected portions of mid-infrared light when their vibrations match the incoming energy. Different chemical components therefore produce distinct absorption patterns. Measuring these patterns converts molecular behavior into contrast, allowing proteins, lipids, carbohydrates, and other constituents to be distinguished within a biological sample or engineered material.
Absorption spectra provide chemical information that spatial intensity alone cannot supply. A spectral feature can indicate where a particular molecular component is concentrated, while its distribution across an image can reveal organization or heterogeneity. This combination of spectral and spatial information helps connect molecular composition with the structure of cells, tissues, biomaterials, or engineered constructs.
Mid-infrared Imaging can generate molecular contrast without requiring fluorescent labels. That distinction is important when the goal is to examine intrinsic chemical composition rather than visualize a selected labeled target. The resulting measurements can support label-free characterization of biological and engineered samples, while the observed contrast arises from absorption associated with molecular vibrations.
Chemical maps can expose compositional differences that may not be apparent from morphology alone. They show how molecular constituents are distributed across a sample, helping relate proteins, lipids, carbohydrates, and other materials to tissue organization or construct architecture. This added chemical dimension is particularly useful when composition and structure change together during disease or biomaterial development.
A typical workflow measures how a sample responds to mid-infrared wavelengths, records wavelength-dependent absorption, and assigns the measured spectral features to spatial locations. The data are then represented as spectral information, chemical maps, or both. This workflow links each region of a cell, tissue, biomaterial, or construct to its local molecular composition.
The method can be applied to cells, tissues, biomaterials, and engineered constructs. In each case, the measurement can characterize molecular composition and its spatial organization without relying on fluorescent labels. This broad sample range makes the approach relevant for comparing biological structures with designed materials and for evaluating how engineered platforms reproduce or alter tissue-associated features.
Chemical maps can track changes in the molecular composition of a biomaterial as it degrades. Comparing the distribution or strength of absorption features provides a way to assess where compositional changes occur within the material or an engineered construct. Such information helps researchers evaluate material performance and relate degradation behavior to the design of regenerative medicine platforms.
In disease-related studies, molecular maps can reveal compositional changes and altered tissue organization. In regenerative medicine, the same information supports the design and evaluation of engineered constructs by showing how their chemical features are arranged. Together, these applications connect molecular measurements with tissue condition, biomaterial behavior, and the performance of platforms intended to support regeneration.